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Coronaviruses

Identification of a recombinant equine coronavirus in donkey, China

, , , , , , , , , , , , , & show all
Pages 1010-1013 | Received 22 Dec 2021, Accepted 17 Mar 2022, Published online: 04 Apr 2022

Figures & data

Figure 1. Comparison between the two Chinese ECoV variants and representative members of Betacoronavirus 1. (A) Pairwise sequence identities between ECoV_2021/464693 and related coronaviruses. The degree of sequence similarity is highlighted by colours, with red denoting the highest identities. For the NS2 gene sequences of different length, they were adjusted according to that of ECoV_2021/464693 (582 nt in length). Phylogenetic trees of the full-length virus genome (B), ORF1ab (C), spike gene (D), and the recombinant region (21,523–22,209 nt) (E). Phylogenetic analysis was performed using RAxML (v8.1.6) with 1000 bootstrap replicates and employing the general time reversible nucleotide substitution model and the GAMMA model for rate distribution. The phylogenetic trees were midpoint rooted. The recombinant region was detected to be 21,523–22,209 nt of the ECoV_2021/464693 genome with RDP (v4).

Figure 1. Comparison between the two Chinese ECoV variants and representative members of Betacoronavirus 1. (A) Pairwise sequence identities between ECoV_2021/464693 and related coronaviruses. The degree of sequence similarity is highlighted by colours, with red denoting the highest identities. For the NS2 gene sequences of different length, they were adjusted according to that of ECoV_2021/464693 (582 nt in length). Phylogenetic trees of the full-length virus genome (B), ORF1ab (C), spike gene (D), and the recombinant region (21,523–22,209 nt) (E). Phylogenetic analysis was performed using RAxML (v8.1.6) with 1000 bootstrap replicates and employing the general time reversible nucleotide substitution model and the GAMMA model for rate distribution. The phylogenetic trees were midpoint rooted. The recombinant region was detected to be 21,523–22,209 nt of the ECoV_2021/464693 genome with RDP (v4).
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