Figures & data
Figure 1. Genome constellation identified in A(H1)v viruses in North America. Genes derived from the following lineages: blue – North American Classical Swine A(H1N1); yellow – North American Avian lineage; green – pre-A(H1N1)pdm09 human seasonal virus; red – Eurasian swine lineage; dashed red box – A(H1N1)pdm09 virus genes; purple – genes derived from A/swine/Texas/4199-2/98 A(H3N2) live attenuated influenza vaccine virus.
Table 1. Comparative hemagglutination inhibition assay assessment of 1A.1.1 (alpha) lineage A(H1N2)v viruses.
Table 2. Comparative hemagglutination inhibition assay assessment of 1B.2 (delta) lineage A(H1N2)v influenza viruses.
Table 3. Summary results of pathogenesis and transmission of A(H1) viruses in the ferret model.
Table 4. Summary results of virus titers in tissues collected on day 3 post inoculation.
Figure 2. Transmission of A(H1) influenza viruses in ferrets. Ferrets were inoculated with 6 log10 PFU of the indicated virus. After 24 h, a naïve ferret was added to each cage housing an inoculated ferret for the Direct Contact Transmission (DCT) model (A-D) or to each cage adjacent to an inoculated ferret for the Respiratory Droplet Transmission (RDT) model (E-K). Nasal wash samples were collected on the days indicated and titered using a standard plaque assay. Virus titers in nasal wash samples collected from individual inoculated ferrets are shown on the left side of each panel while those from individual contact ferrets are shown on the right side of each panel. The limit of detection was 1 log10 PFU/ml (dashed line). *Ferrets were euthanized on day 9 post inoculation or day 11 post contact., respectively due to severe weight loss or dyspnea. ** Nasal wash was collected 8 days post contact., just prior to euthanasia.
