Detection of Bartonella vinsonii subsp. berkhoffii in an HIV patient using metagenomic Next-Generation Sequencing

Figures & data

Figure 1. The diagnosis and treatment of Bartonella vinsonii infection. The lesion around the right knee was shown in (A), and red spots were seen on the right (B) and left leg (C) of the patient. mNGS results were shown in (D) and fluorescent PCR results were displayed in (E), in which blood samples collected from days 11, 13 and 14 after admission were shown as amplification curves 1 (red), 2 (green) and 3 (orange), respectively. Negative control was shown as curve 4 (dark blue). Sanger sequencing alignment was shown in (F). Haematoxylin and eosin (H&E) staining of biopsy tissue was shown in (G). Improvement of the right knee lesion was shown in (H) and the temperature chart of the patient during hospitalization was shown in (I).

Table 1. Laboratory results of the patient after admission.

Laboratory Test	Results	Reference Value
WBC	5.59 × 10^9/L	3.5–9.5 × 10^9/L
Neutrophil count	3.96 × 10^9/L (71%)	40–75%
Lymphocyte count	0.84 × 10^9/L (15%)	20–50%
Monocyte count	0.77 × 10^9/L (13.7%)	3–10%
Haemoglobin	56 g/L	130–175 g/L
RBC	1.92 × 10^12/L	4.3–5.8^12/L
Platelet count	5.0 × 10^9/L	125–350 × 10^9/L
C-reactive protein	94.25 mg/L	0–8 mg/L
Procalcitonin	4.98 ng/mL	0–0.5 ng/mL
HIV serology	Positive	Negative
Treponema pallidum antibody	Positive	Negative
CD4 absolute count	5 cells/µL	410–1590 cells/µL
T-SPOT.TB	Negative	Negative
G test	Negative	Negative
GM test	Negative	Negative
Blood culture	Negative	Negative