Hydroethanolic leaf extract of Acacia auriculiformis exhibited antidiabetic and antioxidant activities

Figures & data

Table 1. Free radical scavenging activities of A. auriculiformis leaf extract/fractions.

Sample	Conc. of sample (µg/ml)	DPPH free radical scavenging (%)	H2O2 free radical scavenging (%)
HEAA	100	14.51 ± 0.13	19.67 ± 0.06
	200	24.54 ± 0.15	31.41 ± 0.24
	300	39.72 ± 0.22	46.44 ± 0.18
	400	48.91 ± 0.10	52.17 ± 0.05
	500	60.43 ± 0.11	64.89 ± 0.12
HFAA	100	00.75 ± 0.14	04.53 ± 0.38
	200	04.37 ± 0.09	10.20 ± 0.17
	300	09.64 ± 0.17	13.73 ± 0.63
	400	12.35 ± 0.19	15.85 ± 0.32
	500	14.74 ± 0.24	19.74 ± 0.24
CFAA	100	01.18 ± 0.07	0.93 ± 0.23
	200	03.18 ± 0.34	07.98 ± 0.29
	300	09.05 ± 0.09	11.84 ± 0.38
	400	16.26 ± 0.15	19.06 ± 0.74
	500	21.68 ± 0.27	24.58 ± 0.27
EFAA	100	10.47 ± 0.25	19.47 ± 0.41
	200	15.48 ± 0.13	28.18 ± 0.16
	300	26.85 ± 0.21	34.75 ± 0.20
	400	42.71 ± 0.19	45.61 ± 0.84
	500	50.87 ± 0.31	59.87 ± 0.31
Standard (Ascorbic acid)	100	83.98 ± 0.047	81.97 ± 0.051
	200	84.70 ± 0.047	83.43 ± 0.051
	300	85.93 ± 0.047	85.52 ± 0.051
	400	87.46 ± 0.047	88.81 ± 0.051
	500	90.05 ± 0.047	90.23 ± 0.051

All readings were taken in triplicate and represented as % ± SEM.

Figure 1. Percentage inhibition of hydroethanolic extract of A. auriculiformis (HEAA) and its fractions against α-amylase.

Figure 2. Percentage inhibition of hydroethanolic extract of A. auriculiformis (HEAA) and its fractions against α-glucosidase.

Table 2. Enzyme inhibition by A. auriculiformis leaf extract and its fractions.

Samples	Conc. of sample (µg/ml)	Percentage inhibition
		α-Amylase	α-Glucosidase	Pancreatic lipase
HEAA	100	18.96 ± 0.00	12.91 ± 0.13	13.68 ± 0.09
	200	39.45 ± 0.18	28.63 ± 0.16	23.40 ± 0.10
	300	51.87 ± 0.16	45.77 ± 0.22	35.67 ± 0.02
	400	59.30 ± 0.31	56.27 ± 0.09	49.86 ± 0.05
	500	61.69 ± 0.05	63.34 ± 0.12	51.77 ± 0.15
HFAA	100	09.45 ± 0.04	06.38 ± 0.14	04.75 ± 0.50
	200	11.21 ± 0.09	12.63 ± 0.09	09.37 ± 0.31
	300	14.45 ± 0.12	16.07 ± 0.17	12.64 ± 0.40
	400	17.88 ± 0.13	19.21 ± 0.19	16.35 ± 0.57
	500	21.32 ± 0.24	25.32 ± 0.47	19.74 ± 0.32
CFAA	100	12.33 ± 0.07	25.15 ± 0.23	1.33 ± 0.19
	200	19.78 ± 0.31	28.34 ± 0.17	7.18 ± 0.34
	300	26.05 ± 0.15	36.47 ± 0.65	11.05 ± 0.09
	400	34.26 ± 0.05	39.08 ± 0.37	18.26 ± 0.15
	500	38.58 ± 0.29	40.87 ± 0.42	27.68 ± 0.27
EFAA	100	19.47 ± 0.45	18.73 ± 0.10	14.28 ± 0.76
	200	24.39 ± 0.23	31.87 ± 0.28	25.26 ± 0.58
	300	38.45 ± 0.31	39.60 ± 0.13	31.85 ± 0.21
	400	46.40 ± 0.19	50.19 ± 0.74	39.71 ± 0.36
	500	53.87 ± 0.33	58.46 ± 0.90	49.87 ± 0.31
Standard (acarbose for α-amylase and α-glucosidase assays; orlistat for pancreatic lipase assay	100	52.34 ± 0.08	42.34 ± 0.10	45.38 ± 0.07
	200	63.03 ± 0.04	56.93 ± 0.02	57.19 ± 0.08
	300	69.71 ± 0.03	58.71 ± 0.07	60.45 ± 0.30
	400	74.49 ± 0.10	62.49 ± 0.05	73.05 ± 0.02
	500	81.26 ± 0.07	79.23 ± 0.15	81.74 ± 0.04

All readings were taken in triplicate and represented as % ± SEM.

Table 3. IC₅₀ value of extract and fractions of A. auriculiformis.

Extract/fraction	IC50 values
	DPPH	H2O2	α-Amylase	α-Glucosidase	Pancreatic lipase
HEAA	4.06	3.63	3.35	3.67	4.47
HFAA	14.57	14.41	14.55	10.66	13.12
CFAA	10.34	9.35	6.55	6.81	8.78
EFAA	4.91	4.26	4.48	4.04	5.07
Standard	1.22	1.34	3.71	4.49	4.21

Figure 3. Percentage inhibition of hydroethanolic extract of A. auriculiformis (HEAA) and its fractions against pancreatic lipase.