The protective effect of Eruca sativa against lipid metabolic abnormalities induced by dioxin in male rats

Figures & data

Table 1. Total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activity (AOA) by DPPH and ABTS of ELSE.

Total phenols (mg GAE/g)	Total flavonoids (mg CE/g)	AOA DPPH (mg TE/g) (%)	AOA ABTS (mg TE/g) (%)
12.522	9.938	2.71 (63.2)	5.548 (90.3)

Gallic acid equivalent (GAE), catechin equivalent (CE), trolox equivalent (TE)

Figure 1. Gas chromatography-mass spectrometry chromatogram of the ELSE.

Table 2. GLC-MS analysis of Fatty acids methyl esters of ELSE.

No	RT	Area	Name	C	Area Sum %
1	16.78	5483.5	Lauric acid	C12:0	8.45
2	20.74	11266	Non identified	– –	17.35
3	22.74	2649.14	Myristic acid	C14:0	4.08
4	28.65	9246.8	Palmitic acid	C16:0	14.24
5	34.25	4251.95	Stearic acid	C18:0	6.55
6	35.09	9249.61	Oleic acid	C18:1	14.25
7	36.83	5306.79	Linoleic acid	C18:2	8.17
8	38.03	1340.42	Gamma-linolenic acid	C18:3	2.06
9	38.95	6100.62	Linolenic acid	C18:3	9.4
10	42.87	1339.42	Homo-γ-linolenic acid	C20:3	2.06
11	43.67	6594.98	Arachidonic acid	C20:4	10.16
12	49.06	2097.07	Lignoceric acid	C24:0	2.23

Table 3. Serum and testicular total cholesterol (CHOL), triacylglycerol (TG), phospholipids (PLs), and total lipids (TL), concentrations in the control and different treated rat groups.

Parameters M±SE	Control	CO	Es	TCDD	Es+ TCDD
Serum CHOL (mg/dl)	75.5±1.90^a	75.38±1.78^a	74.35±1.52^a	109.92±2.34^c	84.03±1.55^b
Testicular CHOL (mg/g)	13.01±0.10^a	13.23±0.12^a	12.57±0.21^a	24.79±0.33^b	15.09±0.48^c
Serum TG (mg/dl)	60.18±0.86^a	60.00±0.89^a	58.00±0.97^a	102.00±1.47^b	76.03±2.48^c
Testicular TG (mg/g)	15.54±0.92^a	15.63±0.76^a	15.23±0.55^a	35.92±1.66^b	20.70±0.37^c
HDL-c (mg/dl)	42.37±1.25^a	42.00±1.63^a	43.58±0.97^a	29.52±0.78^b	38.85±0.76^a
LDL-c (mg/dl)	21.1±0.82^a	21.38±0.55^a	19.17±0.65^a	60.00±1.78^b	29.98±1.24^c
VLDL-c (mg/dl)	12.04±0.17^a	12.00±0.18^a	11.60±0.19^a	20.40±0.29^b	15.21±0.50^c
Serum TL (mg/dl)	334.30±5.75^a	335.27±6.47^a	331.40±6.86^a	498.03±6.82^b	381.77±8.45^c
Testicular TL (mg/g)	58.80±1.36^a	58.72±2.33^a	55.50±0.94^a	106.48±1.21^b	70.32±2.69^c
Serum PLs (mg/dl)	117.18±2.08^a	117.12±1.64^a	115.98±1.98^a	173.53±2.72^b	126.53±1.82^c
Testicular PLs (mg/dl)	23.65±1.50^a	23.83±1.12^a	23.28±0.98^a	45.38±1.30^b	28.20±1.23^a

Each value represents the mean ± SE (n=6) values superscripts with different letters (a-d) were significantly different at p≤0.05

Table 4. Serum and testicular concentration of lactate dehydrogenase (LDH), alkaline phosphatase (ALP), acid phosphatase (ACP), and γ- glutamyl transferase (γ- GT) in the control and in the different treated rat groups.

Parameters M±SE	Control	CO	Es	TCDD	Es+TCDD
Serum ALP (IU/l)	92.72±1.79^a	93.07±2.62^a	91.77±1.56 ^a	182.83±1.71^b	109.32±1.76^c
Testicular ALP activity (U/g)	13.22±0.46^ab	13.07±0.51^a^b	14.22±0.47^a	5.18±0.48^c	11.10±0.47^b
Serum ACP (IU/l)	39.8±0.93^a	40.70±1.21^a	37.6±1.27 ^a	80.05±1.53^b	53.53±1.29^c
Testicular ACP activity (U/g)	2.29±0.18^ab	2.28±0.17^ab	2.64±0.18^a	1.02±0.08^c	1.97±0.16^b
Serum γ-GT (IU/l)	10.28±1.12^a	10.67±0.93^a	9.50±1.08 ^a	27.52±1.84^b	14.92±1.05^a
Testicular γ-GT activity (U/g)	2.31±0.10^ab	2.27±0.10^a^c	2.60±0.08^b	0.93±0.11^d	1.95±0.09^c
Serum LDH (IU/l)	269.35±5.49^a	270.70±3.98^a	266.20±6.58 ^a	429.91±8.09^b	316.15±7.09^c
Testicular LDH activity (U/g)	15.23±0.61^a	15.57±0.63^ab	14.45±0.71^a	31.42±0.91^c	19.68±1.38^b

Each value represents the mean ± SE (n=6) values superscripts with different letters (a-c) were significantly different at p≤0.05