Biochemical and neurosurgical analyses of circulating neuroglobin and somatic mutations in IDH1 gene in patients with traumatic brain injury

Figures & data

Figure 1. Detection of IDH1 c.395 G˃A mutation by RFLP-PCR analysis. Numbers refer to lanes. Lane 1 shows 50 bp DNA ladder. [A] Lane 2 showed PCR amplification product (undigested) with band at 261 bp; Lanes 3–9 showed single band at 237bp corresponding to wild type IDH1. [B] Lane 2–7 showed mutant variant of IDH1 with appearance of two closely placed bands (261 bp and 237 bp).

Table 1. Circulating neuroglobin in patients with traumatic brain injury (TBI) in terms of severity and outcome.

Variable	Patients with TBI (n = 30)	Healthy controls (n = 30)	P value
NGB (median, IQR; pg/mL)	30.06 (5.35–49.16)	7.52 (5.05–25.3)	0.020*
	Patients with mild to moderate TBI (n = 23)	Patients with severe TBI (n = 7)	P value
	18.4 (4.82–41.18)	76.59 (73.11–76.59)	<0.001*
	Patients with TBI and favorable outcome (n = 26)	Patients with TBI and unfavorable outcome (n = 4)	P value
	30.06 (5.35–41.18)	45.76 (7.6–75.719)	0.601

*Significant p value ˂0.05.

Figure 2. Negative correlation between serum NGB levels and GCS, (r = −0.498, p = 0.005).

Figure 3. Negative correlation between serum NGB levels and RTS, (r = −0.521, p = 0.003).

Figure 4. ROC Characteristic Curve for serum neuroglobin (pg/mL) in patients with traumatic brain injury. [A] For predicting the severity of TBI. [B] For predicting the outcome of TBI.

Table 2. Correlation of serum neuroglobin with severity and outcome scores in patients with traumatic brain injury.

Variables		Serum neuroglobin levels (pg/mL)
Glasgow coma scale (GCS)	r	−0.498**
	p	0.005
Revised trauma scale (RTS)	r	−0.521**
	p	0.003
Extended Glasgow outcome scale (GOSe)	r	−0.201
	p	0.288
Disability Rating Scale (DRS)	r	0.227
	p	0.228

Table 3. Genotypes frequencies of IDH1 gene in patients with traumatic brain injury in terms of severity.

Study groups	IDH1 genotypes among the study groups
	Wild type		Mutant type
	No.	%	No.	%
Mild to moderate (n = 23)	7	100	16	69.6
Severe (n = 7)	0	0	7	30.4
P-value (χ ^2)	0.096(2.779)
OR (95%CI)	-

Table 4. Genotypes frequencies of IDH1 gene in patients with traumatic brain injury in terms of outcome.

Study groups	IDH1 genotypes among the study groups
	Wild type		Mutant type
	No.	%	No.	%
Improved (n = 26)	6	85.7	20	87
Died (n = 4)	1	14.3	3	13
P-value (χ ^2)	0.933(0.007)
OR (95%CI)	0.900(0.078–10.327)