Phenotypic and prevalence correlation with recurrent KRAS G12C, G13D, and G22E mutations in colorectal adenocarcinoma

Figures & data

Figure 1. NCBI reference sequence: NG_007524.2.

Figure 2. Agarose gel (1.5%) post-electrophoresis displayed 263 bp PCR products in lanes 2, 3, and 4 for KRAS gene mutation linked to (CRC). Lane 1 had a 100 bp DNA ladder.

Figure 3. g.10653 codon 12 substitution G>T, ggt> tgt Rs121913530 nucleotide sequence (forward) in KRAS gene, exon 2.

Figure 4. g.10658 codon 13 substitution G>A, rs397517040 ggc>gac nucleotide sequence (forward) in KRAS, exon 2.

Figure 5. g.10683 codon 22 cag>gag substitution C>G, nucleotide sequence (forward) in KRAS gene, exon 2.

Table 1. Distribution the KRAS gene with (CRC) study in wild-type and mutation according to age, gender and histological-cell.

Character		Wild-type no. (%)	Mutation no. (%)	Total	Chi-Square: χ^2	P-value
Total		29(16.8)	144(83.2)	173	52.668	0.0001
Age	≤ 40	7 (13.5)	45(86.5)	52	5.628*	0.0388
	˃ 40	22(18)	99(82)	121
Gender	Male	24(20)	96(80)	120	8.722**	0.0074
	Female	5(9.5)	48(90.5)	53
Histological-cell	Well-cell	19(33.3)	38(66.7)	57	5.819*	0.0327
	Moderate-cell	5(5.0)	90(95.0)	95
	Differentiation-cell	5(24)	16(76)	21

*(p ≤ 0.05), **(p ≤ 0.01).

Table 2. Correlation between age, gender and histological cell with KRAS mutation.

Character		Mutation no. 144(83%)			Chi-Square: χ^2	P-value
		Codon 12	Codon 13	Codon 22
Age	≤ 40 No. 45	16 (35.5%)	23(51.1%)	6 (13.4%)	9.705**	0.0054
	˃ 40 No. 99	39 (39.4%)	50 (50.5%)	10 (10.1%)
Gender	Male No. 96	32 (33.3%)	58 (60.4%)	6 (6.3%)	12.341**	0.0006
	Female No. 48	23 (48%)	15 (31.2%)	10 (20.8%)
Histological-cell	Well-cell No. 38	17 (44.7%)	13 (34.3%)	8 (21.0%)	12.074**	0.0015
	Moderate-cell No. 90	27 (30%)	58 (64.4%)	5 (5.6%)
	Differentiation-cell No. 16	11 (68.8%)	2 (12.5%)	3 (18.7%)

**(p ≤ 0.01).

Table 3. Codons 12, 13 and 22 and amino acids change.

Codon	Mutation	Change of mutation	Amino acid change	Percentage	Chi-Square: χ^2	P-value
codon 12	G12C	G/T GGT>TGT	Glycine/Cysteine	55/144 (38.2%)	14.282**	0.0001
codon 13	G13D	G/A GGC>GAC	Glycine/Aspartic Acid	73/144 (50.7%)
codon 22	G22E	C/G CAG>GAG	Glycine/Glutamic Acid	16/144 (11.1%)

**(p ≤ 0.01).