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Computer Science

An automated approach for fibroblast cell confluency characterisation and sample handling using AIoT for bio-research and bio-manufacturing

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Article: 2240087 | Received 13 Mar 2023, Accepted 19 Jul 2023, Published online: 02 Aug 2023

Figures & data

Figure 1. Diagram of the overall system.

Figure 1. Diagram of the overall system.

Figure 2. Sample fibroblast cells images from the dataset with 10x and 4x magnifications.

Figure 2. Sample fibroblast cells images from the dataset with 10x and 4x magnifications.

Figure 3. U-Net architecture used for model training.

Figure 3. U-Net architecture used for model training.

Figure 4. Illustration of the patching-unpatching technique. (a) Original image (b) Patched images (c) Patched images with predictions and (d) Unpatched (reconstructed) image.

Figure 4. Illustration of the patching-unpatching technique. (a) Original image (b) Patched images (c) Patched images with predictions and (d) Unpatched (reconstructed) image.

Figure 5. Illustration of the robotic arm initial parameters.

Figure 5. Illustration of the robotic arm initial parameters.

Figure 6. The prototype of the developed automated cell detection and characterisation using AIoT.

Figure 6. The prototype of the developed automated cell detection and characterisation using AIoT.

Figure 7. (a) Prediction on 4x magnification image and (b) prediction on 10x magnification image.

Figure 7. (a) Prediction on 4x magnification image and (b) prediction on 10x magnification image.

Table 1. Evaluation of the CNN model

Figure 8. Results of confluency estimation for (a) 4x magnification and (b) 10x magnification.

Figure 8. Results of confluency estimation for (a) 4x magnification and (b) 10x magnification.

Table 2. Performance of the AIoT system on different platforms

Figure 9. Schematic diagram of the integrated AIoT system.

Figure 9. Schematic diagram of the integrated AIoT system.

Figure 10. Model training performance graphs.

Figure 10. Model training performance graphs.

Figure 11. Errors caused by the patching-unpatching technique.

Figure 11. Errors caused by the patching-unpatching technique.