Interactive effects of Foliar 24-Epibrassinolide and selenium applications on yield, reduce nitrate accumulation and selenium enrichment in potato tuber in field

Figures & data

Table 1. Effect of 24-epiassinosteroid (EBL) and selenium (Se) foliar application on shoot fresh weight, leaf chlorophyll content, tuber number and yield of potato. RC: Percent change

EBL (µM)	Se (mgl^−1)	Plant fresh weight (gr)	RC	Chlorophyll content (mg/gfw)	RC	Tuber number	RC	Yield (ton/ha)	RC
0	0	679.60^d	100	10.14^ef	100	6.80^d	100	32.60^h	100
	15	680.55^cd	112	10.38^def	102	7.44^cd	109	33.62^g	103
	30	674.22^d	111	9.82^f	97	7.41^cd	109	34.48^g	106
	60	699.05^bc	115	10.07^f	99	7.55^cd	111	34.44^g	106
0.07	0	701.55^bc	115	10.32^def	102	7.55^cd	111	33.88^g	104
	15	697.82^bc	114	11.44^bcde	113	8.41^abc	124	36.14^f	111
	30	704.44^b	116	12.07^abc	119	8.66^abc	127	37.33^e	115
	60	713.55^b	117	11.56^bcd	114	8.69^abc	128	36.71^ef	113
0.1	0	700.55^bc	115	11.01^cdef	109	7.95^abcd	117	36.11^f	111
	15	699.99^bc	115	11.62^bcd	115	8.16^abc	120	37.01^ef	114
	30	706.77^b	116	12.41^ab	122	8.63^abc	127	38.70^d	119
	60	743.53^a	122	11.44^bcde	113	8.69^abc	128	38.76^d	119
0.14	0	698.88b^c	115	11.56^bcd	114	8.96^ab	132	39.41^cd	121
	15	712.99^b	117	12.28^abc	121	9.30^a	137	41.40^a	127
	30	743.39^a	122	13.09^a	129	9.29^a	137	41.06^ab	126
	60	734.76^a	121	12.50^ab	123	9.02^ab	133	40.29^bc	124

Means following same letters within each column do not differ significantly using DMRT at P ≤ 0.05.

Table 2. Effect of 24-epiassinosteroid (EBL) and selenium (Se) foliar application on leaf catalase and superoxide dismutase enzymes, proline, malondialdehyde and tuber alanine, methionine, isoleucine and glycine. RC: Percent change

EBL (µM)	mgl^−1	Catalase (∆OD/g fw min)	RC	Superoxide dismutase (U/mg protein)	RC	Proline (mg/gdw)	RC	malondialdehyde (nmol/cm)	RC	Alanine (mg/g)	RC	Methionine (mg/g)	RC	Isoleucine (mg/g)	RC	Glycine (mg/g)	RC
0	0	12.59^abc	100	8.03^k	100	2.°^3li	100	4.96^a	100	1.92^h	100	1.00^c	100	2.80^m	100	1.99^g	100
	15	12.28^bc	98	8.13^jk	101	2.08^hi	102	4.24^ab	85	2.66^g	139	1.20^bc	120	2.85^l	102	2.37^f	119
	30	11.41^c	91	8.24^j	103	2.30^ef	113	4.59^ab	93	2.85^def	148	1.34^ab	134	3.00^j	107	2.49^ef	125
	60	12.27^bc	97	8.71^gh	109	2.20^g	109	4.42^ab	89	2.82^ef	147	1.27^ab	127	3.05^i	109	2.61^def	131
0.07	0	12.15^bc	97	8.48^i	106	2.13^h	105	4.73^ab	95	2.94^abcd	153	1.24^ab	124	3.01^ij	107	2.49^ef	125
	15	12.99^abc	103	8.65^h	108	2.37^de	117	4.41^ab	89	2.77^f	144	1.25^ab	125	2.91^k	104	2.39^f	120
	30	12.44^abc	99	8.85^fg	110	2.43^bcd	120	4.12^ab	83	2.85^def	148	1.27^ab	127	3.16^h	113	2.86^bcde	144
	60	13.41^ab	107	8.42^i	105	2.29^f	113	4.06^ab	82	2.88^def	150	1.34^ab	134	3.57^d	128	2.99^abcd	150
0.1	0	13.43^ab	107	8.95^f	111	2.24^fg	110	3.96^ab	80	2.91^cde	151	1.34^ab	134	3.27^g	117	2.67^cdef	134
	15	12.09^bc	96	9.29^d	116	2.31^ef	114	3.83^ab	77	2.78^f	145	1.32^ab	132	3.48^e	124	2.72^cdef	136
	30	12.22^bc	97	10.05^c	125	2.40^cd	118	3.71^b	75	2.96^abcd	154	1.27^ab	127	3.56^d	127	3.16^ab	159
	60	12.69^abc	101	10.41^b	130	2.50^ab	123	3.57^b	72	3.03^ab	158	1.39^ab	139	3.63^c	130	3.18^ab	160
0.14	0	13.14^ab	104	8.92^f	111	2.29^f	113	3.62^b	73	3.00^abc	156	1.46^a	146	3.40^f	121	3.39^a	170
	15	13.21^ab	105	9.12^e	114	2.37d^e	117	3.67^b	74	2.86^def	149	1.25^ab	125	3.79^b	135	3.04^abc	153
	30	13.91^a	110	10.42^b	130	2.46^bc	121	3.54^b	71	2.93^bcde	153	1.29^ab	129	3.84^a	137	3.17^ab	159
	60	13.41^ab	106	10.98^a	137	2.54^a	125	3.55^b	72	3.04^a	158	1.36^ab	5136	3.75^b	134	3.20^ab	161

Means following same letters within each column do not differ significantly using DMRT at P ≤ 0.05.

Table 3. Effect of 24-epiassinosteroid (EBL) and selenium (Se) foliar application on tuber nitrate content, leaf nitrate reductase and the content of selenium and starch content of potato tuber. RC: Percent change

EBL (µM)	Se (mgl^−1)	Nitrate content (mg/kg)	RC	Nitrate reductase (µg nitrite/gfw hour)	RC	Selenium content (mg/gdw)	RC	Starch content (%)	RC
0	0	654.86^a	100	9.12^de	100	1.82^e	100	12.68^f	100
	15	600.20^b	92	9.63^d	106	5.29^d	291	13.68^f	108
	30	587.05^b	90	10.76^c	118	7.17^bcd	394	13.74^f	108
	60	573.27^bc	88	11.01^c	121	7.54^bc	414	16.08^de	127
0.07	0	604.88^b	92	9.99^d	109	2.06^e	113	15.52^e	122
	15	534.35^cd	82	10.87^c	119	7.32^bcd	402	16.13^de	127
	30	493.09^de	75	11.42^bc	125	9.17^ab	504	16.97^bcde	134
	60	510.87^de	78	11.88^b	130	8.50^ab	467	16.45^de	130
0.1	0	558.77^bc	85	10.19^cd	112	2.32^e	128	17.24^bcd	136
	15	470.01^e	72	12.36^b	136	5.81^cd	319	17.07^bcde	135
	30	407.92^f	62	12.87^b	141	8.50^ab	467	18.41^ab	145
	60	405.40^f	62	12.75^b	139	10.17^a	559	16.74^cde	132
0.14	0	478.83^e	73	11.04^c	121	2.48^e	136	16.91^bcde	133
	15	417.92^f	64	13.14^ab	144	7.29^bcd	401	18.06^abc	142
	30	403.77^f	62	13.44^a	147	7.51^bc	413	19.07^a	150
	60	389.34^f	61	13.493^a	147	9.83^a	540	18.01^abc	142

Means following same letters within each column do not differ significantly using DMRT at P ≤ 0.05