Antifungal and anti-cellulases activity of Limoniastrum feei extracts to promote Bayoud disease treatment using bioautography

Figures & data

Table 1. Rf values of TLC in different extracts

Eluent	Extraction Solvent	Parts	Rf
EtOAc: Hep 75:25	Hex	Aerial part	0.00	0.57	0.85	0.90	0.95	-
		Roots	0.00	0.54	0.81	0.90	0.93	-
	EtOAc	Aerial part	0.00	0.35	0.57	0.85	0.90	0.95
		Roots	0.00	0.6	0.81	0.90	0.96	-
	DCM	Aerial part	0.00	0.35	0.57	0.85	0.90	0.95
		Roots	0.00	0.54	0.81	0.90	0.93	-
	MeOH	Aerial part	0.00	0.35	0.90	-	-	-
		Roots	0.00	0.81	0.93	-	-	-

Hex: hexane; EtOAc: ethyl acetate; DCM: dichloromethane; MeOH: methanol; Hep: heptane.

Table 2. Phytochemical screening of various extracts of Limoniastrum feei

Extraction Solvent	Parts	Constituents
		Flavonoids	Rf	Tannins	Rf	Coumarins	Rf
Hex	Aerial part	+	0.95	-	-	-	-
	Roots	+	0.00 0.54	-	-	-	-
EtOAc	Aerial part	+	0.00 0.35	+	0.00 0.85 0.95	+	0.95
	Roots	+	0.00 0.6	+	0.00 0.81	+	0.00
DCM	Aerial part	-	-	+	0.95	-	-
	Roots	-	-	+	0.00 0.54	-	-
MeOH	Aerial part	+	0.00 0.35 0.90	-	-	-	-
	Roots	+	0.81 0.93	-	-	-	-

Hex: hexane; EtOAc: ethyl acetate; DCM: dichloromethane; MeOH: methanol; Hep: heptane; Rf: rapport frontal; +: positive; -: negative

Figure 1. Saponins from the stems of L. feei (1 and 2) (Belboukhari & Cheriti, 2009).

1:2α,3β,23-Trihydroxy-30-acetylolean-12-ene (C₃₂H₅₂O₅)

2:1-O-[α-L-Rhamnopyranosyl-(1)-6-O-acetylβ -D-galactopyranosyl]-1 β,3 β,22 ξ − 26-tetrahydroxyfurost-5(6)-ene-26-Oβ -D-glucopyranosid(C₅₀H₈₂O₁₉)

Table 3. Characterization of alkaloids on the TLC plate

Extraction Solvent	Parts	Spots number	Rf	Color
Hex	Aerial part	5	00	Light Yellow
			0.57	Light Brown
			0.85	Green
			0.90	Brown
			0.95	Green
	Roots	5	00	Yellow
			0.54	Purple
			0.81	Brown
			0.90	Purple
			0.93	Light Brown
EtOAc	Aerial part	6	00	Brown
			0.35	Light Purple
			0.57	Purple
			0.85	Light Brown
			0.90	Brown
			0.95	Green
	Roots	5	00	Brown
			0.60	Light Brown
			0.81	Dark Purple
			0.90	Dark Brown
			0.96	Light Brown
DCM	Aerial part	6	00	Brown
			0.35	Brown
			0.57	Green
			0.85	Brick red
			0.90	Light Green
			0.95	Green
	Roots	5	00	Brown
			0.54	Light Brown
			0.81	Light Purple
			0.90	Brown
			0.93	Brown
MeOH	Aerial part	3	00	Brown
			0.35	Yellow
			0.90	Green
	Roots	3	00	Pink
			0.81	Light Pink
			0.93	Yellow

Hex: hexane; EtOAc: ethyl acetate; DCM: dichloromethane; MeOH: methanol; Rf: rapport frontal.

Table 4. Result of the direct bioautography of the plant extracts

Eluent	Extraction Solvent	Parts	Antifungal effect
EtOAc: Hep 75:25	Hex	Aerial part	-
		Roots	+
	EtOAc	Aerial part	+
		Roots	+
	DCM	Aerial part	-
		Roots	-
	MeOH	Aerial part	+
		Roots	+

Hex: hexane; EtOAc: ethyl acetate; DCM: dichloromethane; MeOH: methanol; Hep: heptane; Rf: rapport frontal.

Table 5. Effect of L. feei extracts on Fusarium oxysporum f. sp. albedinis cellulases using contact bioautography

Eluent	Extraction Solvent	Parts	Number of IZ	Inhibition zone
				Rf	Ø
EtOAc: Hep 75:25	Hex	Aerial part	00	-	-
		Roots	01	0.54	2
	EtOAc	Aerial part	01	0.35	2
		Roots	00	-	-
	DCM	Aerial part	00	-	-
		Roots	00	-	-
	MeOH	Aerial part	01	00	5
		Roots	00	-	-

IZ: Inhibition zone; Hex: Hexane, EtOAc: Ethyl acetate, DCM: Dichloromethane, MeOH: Methanol; Hep: Heptane; Ø: diameter in mm

Figure 2. Flavonoids from the stems of L. feei (1–4) (Belboukhari & Cheriti, 2007).

(1): 6, 3ʹ, 4ʹ-Tri-methoxy 3, 5, 5ʹ-trihydroxy flavonol.

(2): 3-(6”-malonyl 2”-ramnosyl glucosil) 6, 3ʹ, 4ʹ-tri-methoxy 5, 5ʹ-dihydroxy flavonol.

(3): Tetraacetate 7-dihydroxy-4ʹ-Methoxy 8-O-β-glucopyranoside isoflavone.

(4): Tetraacetate 7, 4ʹ-diMethoxy 8-O-β-glucopyranoside isoflavone

Belboukhari, N., & Cheriti, A. (2009). Analysis and isolation of saponins from Limoniastrum feei by LC-UV. Chemistry of Natural Compounds, 45(5), 756. doi:10.1007/s10600-009-9455-2

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Belboukhari, N., & Cheriti, A. (2007). Flavonoids of Limoniastrum feei. Research Journal of Phytochemistry, 1(2), 74–78. doi:10.3923/rjphyto.2007.74.78

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