Occurrence of cassava mosaic begomovirus-associated satellites on cassava in Ghana

Figures & data

Figure 1. Areas surveyed for cassava mosaic begomoviruses and associated DNA satellites

Table 1. Primers, primer sequences and their respective target viruses used for the Cassava mosaic begomovirus and associated satellites

Target virus, satellite	Primer	Primer sequence (5´- 3´) Reference
ACMV	JSP001	ATGTCGAAGCGACCAGGAGAT Fondong et al., 2000
	JSP002	TGTTTATTAATTGCCAATACT Fondong et al., 2000
EACMV-CM	JSP001	ATGTCGAAGCGACCAGGAGAT Fondong et al., 2000
	JSP003	CCTTTATTAATTTGTCACTGC Fondong et al., 2000
satII	Sat IIF	GCCGCACCACTGGATCTC Ndomba, 2012
	Sat IIR	CAGCAGCCAGTCAGGAAGTT Ndomba, 2012
satIII	Sat IIIF	AGGCCTCGTTACTAAAAGTGC Ndomba, 2012
	Sat IIIR	ACCTGACGGCAGAAGGAAT Ndomba, 2012

Synthesized by Inqaba Biotech West Africa Ltd, Ibadan, Nigeria.

Figure 2. Cassava leaves. (a), Asymptomatic (b), Moderately severe mosaic symptom (c), Severe mosaic symptom (d). Very severe mosaic symptom

Figure 3. Image of electrophoresis of total PCR product on 1.5% agarose gel generated using primer pair JSP001/002 that detect ACMV. M-1.0 kb molecular marker; Lanes represent CMD-affected leaf samples collected from the various locations in southern Ghana

Figure 4. Agarose gel electrophoresis of DNA satIII specific PCR products of 306 bp from CMD-affected cassava leaf samples total nucleic acid. Lanes 1 to 14 contained separate cassava samples. M-1.0 kb molecular marker; Lane 15- Negative Control

Figure 5. Agarose gel electrophoresis of DNA satII specific PCR products of 895 bp from CMD-affected cassava leaf samples total nucleic acid. Lanes 1 to 14 . Lanes 1 to 14 contained separate cassava samples. M-1.0 kb molecular marker; Lane 15- Negative Control

Fondong, V. N., Pita, J. S., Rey, M. E., De Kochko, A., Beachy, R. N., & Fauquet, C. M. (2000). Evidence of the synergism between African cassava mosaic virus and a new double-recombinant geminivirus infecting cassava in Cameroon. Journal of General Virology, 81 (1), 287–297. DOI:https://doi.org/10.1099/0022-1317-81-1-287

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Ndomba, A. O. (2012). ‘Influence of satellite DNA molecules severity of cassava begomoviruses and the breakdown of resistance to cassava mosaic disease in Tanzania [PhD Thesis]. University of the Witwatersrand.

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