In vitro activity of soybean extract on cocoa disease pathogens (Erythricium salmonicolor and Marasmiellus scandens) and termite pest (Microtermes subhyalinus)

Figures & data

Figure 1. Effect of different concentrations of soybean extract on the mycelial growth of E. salmonicolor (ES) at 5 and 7 days of incubation (28 ± 2°C). Values of percentage inhibition bearing the same letter within the same incubation day are not significantly different at p < 0.05 using Tukey’s post hoc test.

Figure 2. Effect of different concentrations of soybean extract on the mycelial growth of Marasmiellus scandens (MS) isolates at 5 and 7 days of incubation (28 ± 2°C). Values of percentage inhibition bearing the same letter within the same incubation day are not significantly different at p < 0.05 using Tukey’s post hoc test.

Figure 3. Binary logistic model of soybean extract on the mycelial growth of isolates of Erythricium salmonicolor (ES) after different incubation days. A: ES 1 after 5 days of incubation B: ES 1 after 7 days of incubation C: ES 2 after 5 days of incubation D: ES 2 after 7 days of incubation E: ES 3 after 5 days of incubation F: ES 3 after 7 days of incubation.

Figure 4. Binary logistic model of soybean extract on the mycelial growth of isolates of Marasmiellus scandens (MS) after different incubation days. A: MS 1 after 5 days of incubation B: MS 1 after 7 days of incubation C: MS 2 after 5 days of incubation D: MS 2 after 7 days of incubation E: MS 3 after 5 days of incubation F: MS 3 after 7 days of incubation.

Table 1. Inhibition activity of soybean extract on isolates of Erythricium salmonicolor and Marasmiellus scandens after 5 days of incubation

Fungi	IC50 (%v/v) [95% CI]	Slope ± SE	Intercept ± SE
Erythricium salmonicolor 1 (ES1)	2.4370^a [1.8691–3.1772]	0.4574 ± 0.0227	0.4074 ± 0.0607
Erythricium salmonicolor 2 (ES2)	1.3406^b [1.0647–1.6881]	0.5398 ± 0.0247	0.1583 ± 0.634
Erythricium salmonicolor 3 (ES3)	0.6428^c [0.5013–0.8241]	0.4796 ± 0.0216	0.2120 ± 0.0618
Marasmiellus scandens 1 (MS1)	0.1193^d [0.0838–0.1699]	0.3015 ± 0.0163	0.6409 ± 0.595
Marasmiellus scandens 2 (MS2)	0.2929^e [0.2141–0.4008]	0.3442 ± 0.0173	0.4226 ± 0.586
Marasmiellus scandens 3 (MS3)	0.4500^ce [0.3468–0.5841]	0.446 ± 0.0202	0.3563 ± 0.0615

CI: Confidence Interval. IC: Inhibition Concentration. SE: Standard Error. IC₅₀ values with the same alphabet in the same column are not significantly different at p < 0.05.

Table 2. Inhibition activity of soybean extract on isolates of Erythricium salmonicolor and Marasmiellus scandens after 7 days of incubation

Fungi	IC50 (%v/v) [95% CI]	Slope ± SE	Intercept ± SE
Erythricium salmonicolor 1 (ES1)	4.6941^a [3.8393–5.7391]	0.6760 ± 0.0350	1.0453 ± 0.0816
Erythricium salmonicolor 2 (ES2)	1.9425^b [1.6104–2.3432]	0.7517 ± 0.0350	0.4991 ± 0.0763
Erythricium salmonicolor 3 (ES3)	1.9425^b [1.5812–2.3864]	0.6433 ± 0.0299	0.4272 ± 0.0698
Marasmiellus scandens 1 (MS1)	0.0735^c [0.05295–0.1019]	0.3418 ± 0.0172	0.8925 ± 0.0643
Marasmiellus scandens 2 (MS2)	1.0459^d [0.8195–1.3350]	0.945 ± 0.0226	0.0222 ± 0.0615
Marasmiellus scandens 3 (MS3)	1.1590^d [0.9494–1.4148]	0.6856 ± 0.0305	0.1012 ± 0.0701

CI: Confidence Interval. IC: Inhibition Concentration. SE: Standard Error. IC₅₀ values with the same alphabet in the same column are not significantly different at p < 0.05.

Figure 5. Binary logistic model of soybean extract on Microtermes subhyalinus after exposure for 24 hours.

Table 3. Mortality effect of soybean extract on Microtermes subhyalinus after 24 hours of exposure

Conc (% v/v)	*Mortality (%)	LC50 (%) [95% CI]	Slope ± SE	Intercept ± SE
50	100.00 ± 0.00^e	15.418 [14.219–16.718]	1.3747 ± 0.0801	−3.7606 ± 0.2575
40	70.00 ± 5.48^d
30	60.00 ± 4.47 ^cd
20	56.00 ± 4.00^c
10	42.00 ± 2.00^b
0	2.00 ± 2.00^a

Conc: Concentration. CI: Confidence Interval. LC: Lethal Concentration. SE: Standard Error. *: Untransformed data. Mortality values with the same alphabet are not significantly different at p < 0.05 using Tukey post hoc test after arcsine-transformation.