Phenotypic characterization of rice Bipolaris oryzae in Fogera plains

Figures & data

Figure 1. Locations of rice brown spot isolates collection.

Table 1. Description of collected B. oryzae isolates.

S.No	Isolates	Place of collection		Sample type	Variety
		District	Kebele Administrations (KAs)
1	MCBO 4	Libokemkem	Shina Tsion	Leaf	Gumara
2	MCBO 6 A	Libokemkem	Shina Tsion	Leaf	Gumara
3	MCBO 6B	Libokemkem	Shina Tsion	Leaf	Gumara
4	MCBO 6 C	Libokemkem	Shina Tsion	Leaf	Gumara
5	MCBO 8	Fogera	Tiwa Zakna	Leaf	Gumara
6	MCBO 9 A	Dera	Zara	Leaf	X-Jigna
7	MCBO 9B	Dera	Zara	Leaf	X-Jigna
8	MCBO 10 A	Dera	Zara	Leaf	X-Jigna
9	MCBO 10B	Dera	Zara	Leaf	X-Jigna
10	MCBO 10 C	Dera	Zara	Leaf	X-Jigna
11	MCBO 13 A	Dera	Jigna	Leaf	Gumara
12	MCBO 13B	Dera	Jigna	Leaf	Gumara
13	MCBO 14 A	Dera	Jigna	Leaf	Gumara
14	MCBO 14B	Dera	Jigna	Leaf	Gumara
15	MCBO 15 A	Dera	Jigna	Leaf	Gumara
16	MCBO 15B	Dera	Jigna	Leaf	Gumara
17	MCBO 15 C	Dera	Jigna	Leaf	Gumara
18	MCBO 23 A	Fogera	Quhar Mikael	Leaf	X-Jigna
19	MCBO 23B	Fogera	Quhar Mikael	Leaf	X-Jigna
20	MCBO 32	Fogera	On station	Leaf	Genotype
21	MCBO 33	Fogera	On station	Leaf	Getachew
22	MCBO 38	Fogera	Abua Kokit	Leaf	X-Jigna
23	MCBO 39 A	Fogera	Abua Kokit	Leaf	X-Jigna
24	MCBO 39B	Fogera	Abua Kokit	Leaf	X-Jigna
25	MCBO 40 A	Fogera	Abua Kokit	Leaf	X-Jigna
26	MCBO 40B	Fogera	Abua Kokit	Leaf	X-Jigna
27	MCBO 41 A	Fogera	Abua Kokit	Leaf	Gumara
28	MCBO 41B	Fogera	Abua Kokit	Leaf	Gumara
29	MCBO 48	Fogera	Tiwa Zakina	Leaf	Gumara

Figure 2. Sample of brown spot and isolation process.

Figure 3. Preparation of spore suspension.

Table 2. Groups of B. oryzae isolates by colony growth pattern and colour.

Group	Colony morphology	Number of isolates	Percentage of isolates (%)	Code of isolates in the group
1	Grey with suppressed	4	13.79	MCBO 4, MCBO 6B, MCBO 15 C, and MCBO 41B
2	Grey with suppressed and white spots	4	13.79	MCBO 6 A, MCBO 14 A, MCBO 23B and MCBO 39 A
3	Grey with fluffy white cottony	5	17.24	MCBO 8, MCBO 9 A, MCBO 10B, MCBO14B, and MCBO 40B
4	Black with fluffy	10	34.48	MCBO 10 A, MCBO 10 C, MCBO 13 A, MCBO 15 A, MCBO 23 A, MCBO 32, MCBO 33, MCBO 38, MCBO 41 A and MCBO 48
5	Black with suppressed and white spots	2	6.89	MCBO 6 C and MCBO 13B
6	Black with suppressed	1	3.45	MCBO 39B
7	Black with fluffy and white spots	1	3.45	MCBO 40 A
8	Yellowish grey with suppressed	1	3.45	MCBO 15B
9	White with cottony growth	1	3.45	MCBO 9B

Figure 4. Colony morphology of B. oryzae isolates.

Table 3. Morphological variability of B. oryzae isolates on artificial growth media.

Isolates	Colony diameter 7 DI (mm)	Colony morphology (growth pattern + colour)	Mycelial growth	Colony Margin/Edge	Colony growth habit	Radial growth (mm)		Luster
						3 DI	7 DI
MCBO 4	52.5	Grey with suppressed	Flat	Irregular	Moderate	17.50	26.25	Shinning
MCBO 6 A	65.0	Grey with suppressed and white spots	Flat	Regular	Moderate	19.25	32.50	Shinning
MCBO 6B	65.0	Grey with suppressed	Flat	Regular	Moderate	19.25	32.50	Shinning
MCBO 6 C	67.5	Black with suppressed and white spots	Cottony	Regular	Moderate	18.50	32.75	Shinning
MCBO 8	85.0	Grey with fluffy white cottony	Cottony	Regular	fast	26.25	42.50	Dully white
MCBO 9 A	78.0	Grey with fluffy white cottony	Cottony	Regular	fast	18.75	39.00	Dully white
MCBO 9B	60.0	White with cottony	Cottony	Regular	Moderate	23.25	30.00	Dully white
MCBO 10 A	90.0	Black with fluffy	Cottony	Regular	fast	35.00	45.00	Dully brown
MCBO 10B	80.0	Grey with fluffy white cottony	Cottony	Regular	fast	22.25	40.00	Shinning
MCBO 10 C	80.0	Black with fluffy	Cottony	Irregular	fast	18.75	40.00	Dully brown
MCBO 13 A	57.0	Black with fluffy	Cottony	Irregular	Moderate	19.50	28.50	Dully brown
MCBO 13B	64.0	Black with suppressed and white spots	Cottony	Regular	Moderate	17.50	32.00	Dully brown
MCBO 14 A	83.5	Greyish suppressed and white spots	Cottony	Regular	fast	22.25	41.75	Dully white
MCBO 14B	80.0	Greyish fluffy white cottony	Cottony	Regular	fast	27.50	40.00	Dully white
MCBO 15 A	72.5	Black with fluffy	Cottony	Irregular	fast	20.25	36.25	Dully brown
MCBO 15B	25.0	Yellowish grey with suppressed	Cottony	Irregular	Slow	10.25	12.50	Dully brown
MCBO 15 C	70.0	Greyish with suppressed	Flat	Regular	Moderate	16.50	35.00	Dully brown
MCBO 23 A	64.0	Black with fluffy	Cottony	Regular	Moderate	18.75	32.00	Dully brown
MCBO 23B	65.0	Grey with suppressed and white spots	Flat	Regular	Moderate	18.25	32.50	Shinning
MCBO 32	75.0	Black with fluffy	Cottony	Irregular	fast	20.25	37.50	Dully brown
MCBO 33	43.5	Black with fluffy	Cottony	Irregular	Moderate	15.75	21.75	Dully brown
MCBO 38	90.0	Black with fluffy	Cottony	Regular	fast	24.00	45.00	Dully brown
MCBO 39 A	66.0	Greyish with suppressed and white spots	Cottony	Regular	fast	21.50	33.00	Dully white
MCBO 39B	75.0	Black with suppressed	Flat	Regular	fast	18.25	37.50	Dully brown
MCBO 40 A	90.0	Black with fluffy and white spots	Cottony	Regular	fast	25.00	45.00	Dully brown
MCBO 40B	72.5	Grey with fluffy white cottony	Cottony	Regular	fast	19.00	36.25	Dully white
MCBO 41 A	48.5	Black with fluffy	Cottony	Irregular	Slow	15.50	24.25	Dully brown
MCBO 41B	69.5	Grey with suppressed	Cottony	Regular	Moderate	16.75	34.75	Dully white
MCBO 48	40.5	Black with fluffy	Cottony	Irregular	Slow	16.00	20.25	Dully brown

DI: Days after inoculation.

Figure 5. A: Conidia length and width of MCBO 10B, B: conidiophore of B. oryzae.

Figure 6. Hilum and mycelia septation of B. oryzae (isolate MCBO 6 C).

Table 4. Characteristics of B. oryzae conidia and dry mycelial weight.

Isolates	Conidia colour	Conidia shape	Spore length (µm)	Spore breadth (µm)	Number of septation/conidium	Mean dry mycelial weight (mg)	Sporulation conidia/ml (µm)
MCBO 4	Brown	Curved	12.08	4.25	3–4	43.5	1.52 × 10^9
MCBO 6 A	Brown	Curved	10.28	2.94	2–4	49	2.34 x 10^9
MCBO 6B	Light brown	Slightly curved	6.88	2.73	3–4	5.5	1.72 x 10^9
MCBO 6 C	Brown	Curved	8.96	2.78	4–5	81	2.72 × 10^9
MCBO 8	Light brown	Curved	6.49	1.88	2–3	59.5	2.76 × 10^9
MCBO 9 A	Light brown	Slightly curved	5.74	2.33	2–4	19	2.20 × 10^9
MCBO 9B	Light brown	Slightly curved	15.49	2.65	3	56	6.00 × 10^9
MCBO 10 A	Brown	Slightly curved	8.02	2.95	3–4	32.5	3.48 × 10^9
MCBO 10B	Brown	Curved	4.96	2.89	3	43	5.41 × 10^9
MCBO 10 C	Light brown	Slightly curved	7.69	1.18	4	50	2.40 × 10^9
MCBO 13 A	Brown	Curved	10.72	3.65	2–3	12.5	3.88 × 10^9
MCBO 13B	Brown	Slightly curved	10.03	3.13	2–5	19.5	2.56 × 10^9
MCBO 14 A	Light brown	Slightly curved	9.47	3.05	3–4	51	1.068 × 10^10
MCBO 14B	Brown	Slightly curved	3.07	1.00	1–3	55	2.05 × 10^9
MCBO 15 A	Brown	Curved	10.30	1.27	3	44.5	5.42 × 10^9
MCBO 15B	Light brown	Curved	13.27	3.48	2–3	49	9.84 × 10^9
MCBO 15 C	Brown	Slightly curved	8.23	3.75	2–3	41.5	9.26 × 10^9
MCBO 23 A	Brown	Slightly curved	9.74	3.68	3–4	45.5	2.96 × 10^9
MCBO 23B	Light brown	Slightly curved	10.36	1.81	2–4	54.5	2.00 × 10^9
MCBO 32	Brown	Slightly curved	8.97	2.85	2–3	8.5	4.12 × 10^9
MCBO 33	Brown	Slightly curved	11.66	3.45	3	27	3.94 × 10^9
MCBO 38	Brown	Slightly curved	14.26	4.21	3–4	31.5	3.08 × 10^9
MCBO 39 A	Light brown	Curved	11.63	2.86	2–3	26	4.04 × 10^9
MCBO 39B	Brown	Slightly curved	11.41	3.03	3	15	3.24 × 10^9
MCBO 40 A	Brown	Curved	14.36	4.27	3–5	35	2.86 × 10^9
MCBO 40B	Light brown	Slightly curved	6.98	3.21	2–4	49.5	1.156 × 10^10
MCBO 41 A	Brown	Slightly curved	10.78	3.29	2–3	32.5	4.00 × 10^9
MCBO 41B	Light brown	Slightly curved	7.68	3.35	3	44.5	1.80 × 10^10
MCBO 48	Light brown	Slightly curved	9.43	3.38	2–3	41.5	3.20 × 10^9

Figure 7. Preservation of B. oryzae isolates mycelia in test tubes.

Table 5. Clusters of B. oryzae isolates collected from three districts based on morphological characters.

Cluster	Number of isolates	Isolate’s name
1	5	MCBO 4, MCBO 15B, MCBO 33, MCBO 41 A and MCBO 48
2	14	MCBO 6 A, MCBO 8, MCBO 10 A, MCBO 13 A, MCBO 13B, MCBO 14 A, MCBO 14B, MCBO 15 A, MCBO 15 C, MCBO 23 A, MCBO 38, MCBO 40 A, MCBO 40B, and MCBO 41B
3	7	MCBO 6B, MCBO 9 A, MCBO 10B, MCBO 10 C, MCBO 32, MCBO 39 A and MCBO 39B
4	3	MCBO 6 C, MCBO 9B and MCBO 23B

Table 6. Cluster means of some morphological parameters of B. oryzae.

Isolate parameters	Cluster means
	1	2	3	4
CD (mm)	42	78	68.57	64.17
CL (µm)	11.44	8.68	9.34	11.60
CW (µm)	3.57	2.83	2.94	2.41
DMW (mg)	38.70	45.14	15.14	63.83

CD (mm): colony diameter in millimeters; CL (µm): conidia length in micrometers; CW (µm): conidia width in micrometers; DMW (mg): dry mycelial weight in milligrams.

Figure 8. Dendrogram showing similarity among isolates of B. oryzae.

Data availability statement

The authors confirm that the data supporting the findings of this study are available within the article.