Chemical composition and antimicrobial activity of leave extract of Ocimum lamiifolium (Damakese) as a treatment for urinary tract infection

Figures & data

Table 1. Chemical components of Ocimum lamiifolium leaf essential oil

Compound name	RT (min)	Mw (g/mol)	Characteristic ions (m/z)	Relative content (area %)
3-Octanol	6.40	130	129, 112, 101, 83, 59, 41	2.66
1-Octen-3-ylpropionate	7.64	184	155, 128, 81, 57, 41	20.82
Linalol	9.03	154	153, 136, 121, 93, 71, 41	28.52
3,7,11-Trimethyl-(E,E)-2,6,10-dodecatrienal	11.07	220	220, 107, 84, 41	12.14
2-Methylphenyl p-methoxybenzoate	11.30	242	242, 152, 135, 77	2.58
7-Methylene bicyclo[3,3,1]nonan-3-ol	12.40	152	152, 134, 119, 92, 79, 41	7.03
2,4,5,6,7,7-Hexahydro-4,7-methano-1H-indene	13.63	134	134, 119, 91,77	6.75
2-Methoxy-4-(1-propenyl)-phenol	15.77	164	164, 121, 91, 134, 105, 91	4.33
δ-Cadinene	19.80	204	204, 161, 134, 105, 91	5.01
α-Terpineol	20.44	154	154, 136, 121, 94, 79, 55	3.72

Figure 1. Chromatogram of Ocimum lamiifolium leaf essential oil.

Table 2. Phytochemical components of ethanol extract

Test	Result
Anthraquinones	-
Terpenoids	+
Flavonoids	+
Saponins	+
Tannins	+
Alkaloids	-
Steroids	+

Table 3. Inhibition zone of ethanol extract and hydrodistilled essential oil

Samples	Concentration of samples	Inhibition zone(mm)
Crude extract	0.05 mg/mL	7 ± 0.50
	0.10 mg/mL	8 ± 0.50
	0.20 mg/mL	9 ± 1.00
	0.40 mg/mL	9 ± 2.00
	0.50 g/mL	10 ± 0.50
Essential oil	0.10 (V/V)	8 ± 1.00
	0.20 (V/V)	9 ± 2.00
	0.40 (V/V)	11 ± 2.00
	0.80 (V/V)	12 ± 1.00
	1.00 (V/V)	12.5 ± 1.50
Chloroform	1.00(V/V) Negative control	0
Ampicillin	Standard (positive control)	24