Chemical profiling and total thickness-excised wound-healing activity of Pistacia lentiscus L. fruits growing in Algeria

Figures & data

Table 1. Oil and protein contents of P. lentiscus L. fruits

Species	Protein (%)	Oil (%) hexane extract	Oil (%) cold pressure extract
P. lentiscus L.	23.856 ± 1.260	25.671 ± 0.852	20.84 ± 1.318

Note: Values presented are the means of triplicate experiments for each extract.

Table 2. Fatty acid analysis results of hexane and cold pressure extracts of P. lentiscus L. by GC–MS

Rt (min)^a	Constituents^b		Hexane extract^c (%)	Cold pressure^c (%)
25.78	Stearic acid, methyl ester	(C18:0)	1.38 ± 0.34	1.29 ± 0.07
18.60	Myristic acid, methyl ester	(C14:0)	0.05 ± 0.02	0.04 ± 0.02
19.83	Pentadecanoic acid, methyl ester	(C15:0)	0.01 ± 0.01	0.01 ± 0.01
21.94	Palmitic acid, methyl ester	(C16:0)	25.75 ± 0.82	25.44 ± 0.65
23.86	Heptadecanoic acid, dimethyl ester	(C17:0)	0.08 ± 0.02	0.06 ± 0.02
29.22	Arachidic acid, methyl ester	(C20:0)	0.21 ± 0.02	0.15 ± 0.02
	∑ SFA		27.48 ± 0.21^a	26.99 ± 0.13^a
22.57	Palmitoleic acid, methyl ester	(C16:1)	4.24 ± 0.12	3.74 ± 0.02
24.43	10-Heptadecanoic acid, methyl ester	(C17:1)	0.08 ± 0.06	0.09 ± 0.01
26.38	Oleic acid, methyl ester	(C18:1)	46.91 ± 1.03	48.34 ± 0.07^a
29.67	11-Eicosenoic acid, methyl ester	(C20:1)	0.15 ± 0.01	0.19 ± 0.01
	∑ MUFA		51.38 ± 0.31^a	52.36 ± 0.03^a
27.26	Linoleic acid, methyl ester	(C18:2)	20.59 ± 0.03	20.27 ± 0.81
28.46	Linolenic acid, methyl ester	(C18:3)	0.55 ± 0.04	0.39 ± 0.01
	∑ PUFA		21.14 ± 0.03^a	20.66 ± 0.41^a
	SFA/UFA		0.38	0.37
	PUFA/SFA		0.77	0.76
	Cox value		2.71	2.65

Note: Values presented are the means of triplicate experiments for each extract.

^a Retention time (in minutes).

^b Compounds listed of elution from a HP-5 MS column.

^c Percentage of relative weight.

Table 3. Evolution of the healing process (contraction %) of total thickness-excised wounds on rabbits

Treatment	Wound healing (%)
	Day 3	Day 6	Day 9	Day 12	Day 15
CONT (-)	24.62 ± 1.41	36.80 ± 4.10	60.80 ± 1.64	81.73 ± 2.99	92.68 ± 1.46
MAD	38.47 ± 2.08	50.73 ± 1.30	72.68 ± 1.33	93.05 ± 3.70	98.50 ± 0.76
PLHE	41.11 ± 1.42	54.64 ± 1.28	70.85 ± 1.53	92.93 ± 4.18	95.29 ±1.39
PLW	18.04 ± 3.26	41.08 ± 2.03	60.30 ± 1.25	79.93 ±1.23	85.38 ± 0.55
Statistical data
PLHE–CONT-	Signif-p ≤ 0.05*	Signif-p ≤ 0.01**	Signif-p ≤ 0.05*	Signif-p ≤ 0.05*	Nonsignificant
PLHE–PLW	Signif-p ≤ 0.05*	Signif-p ≤ 0.05*	Signif-p ≤ 0.05*	Signif-p ≤ 0.01**	Signif-p ≤ 0.05*
PLHE–MAD	Nonsignificant	Nonsignificant	Nonsignificant	Nonsignificant	Nonsignificant
MAD–CONT-	Signif-p ≤ 0.05*	Signif-p ≤ 0.05*	Signif-p ≤ 0.05*	Signif-p ≤ 0.05*	Signif-p ≤ 0.05*
PLW–CONT-	Nonsignificant	Nonsignificant	Nonsignificant	Nonsignificant	Nonsignificant
MAD–PLW	Signif-p ≤ 0.05*	Signif-p ≤ 0.05*	Signif-p ≤ 0.05*	Signif-p ≤ 0.01**	Signif-p ≤ 0.001***

Note: The results represent the mean ± SD (N = 4).