Differential biochemical response of rice (Oryza sativa L.) genotypes against rice blast (Magnaporthe oryzae)

Figures & data

Table 1. Grade description for screening against M. oryzae

0	No lesions observed
1	Small brown specks of pinpoint size or larger brown specks without sporulating center
2	Small roundish to slightly elongated, necrotic gray spots, about 12 mm in diameter with a distinct brown margin
3	Lesion type is the same as in scale 2, but a significant number of lesions are on the upper leaves
4	Typical susceptible blast lesions 3 mm or longer, infecting less than 4% of the leaf area
5	Typical blast lesions infecting 4–10% of the leaf area
6	Typical blast lesions infection 11–25% of the leaf area
7	Typical blast lesions infection 26–50% of the leaf area
8	Typical blast lesions infection 51–75% of the leaf area
9	More than 75% leaf area affected

Table 2. Chromosome location (CL), size range (SR), primers sequence and annealing temperature (AT) for two SSR markers used in the study

S. no	Marker SSR	CL	Forward sequence	Reverse sequence	AT (°C)	SR (bP)
1	RM144	11	5′TGCCCTGGCGCAAATTTGATCC-3′	5′-GCTAGAGGAGATCAGATG GTAGTGCATG-3′	55	214–255
2	RM224	11	5′-ATCGATCGATCTTCACGAGG-3′	5′TGCTATAAAAGGCATTCGGG-3′	55	119–155

Source: Jia and Moldenhauer (2010).

Table 3. Leaf blast severity were recorded by following 0–9 SES scale as per IRRI, Philippines

Genotypes	Score	Percent disease severity	Reaction
KJT-5-1-10-22-38-13	2	2.5	Resistant
KJT-2	3	3.87	Moderately resistant
Tetep	2	2.77	Resistant
Swarnadhan	2	2.70	Resistant
CN-1447-9-4-7	3	3.33	Moderately resistant
NLR-20104	1	1.01	Resistant
RAU-631-9-10	2	1.93	Resistant
CB-06-555	2	2.11	Resistant
RP-Biopatho-3	2	2.00	Moderately resistant
EK-70	9	77	Highly susceptible
Chimansal	9	80	Highly susceptible

Source: Anonymous (2002).

Figure 1. Activity profile of superoxide dismutase (SOD).

Notes: The vertical bar in the figures indicates activity profile of antioxidative enzymes from disease free (control) and after challenge to inoculation with M. oryzae (infected) leaf samples of 11 rice genotypes. The bar indicates SE ± of mean (n = 3) at p < 0.05 probability.

Figure 2. Activity profile of peroxidase (POX).

Notes: The vertical bar in the figures indicates activity profile of antioxidative enzymes from disease free (control) and after challenge to inoculation with M. oryzae (infected) leaf samples of 11 rice genotypes. The bar indicates SE ± of mean (n = 3) at p < 0.05 probability.

Figure 3. Activity profile of ascorbate peroxidase (APX).

Notes: The vertical bar in the figures indicates activity profile of antioxidative enzymes from disease free (control) and after challenge to inoculation with M. oryzae (infected) leaf samples of 11 rice genotypes. The bar indicates SE ± of mean (n = 3) at p < 0.05 probability.

Figure 4. Activity of phenylalanine ammonia lyase (PAL).

Notes: The vertical bar in the figures indicates activity profile of defense-related enzymes from disease free (control) and after challenge to inoculation with M. oryzae (infected) leaf samples of 11 rice genotypes. The bar represent standard error of mean (n = 3) at p < 0.05 probability.

Figure 5. Activity of Chitinase.

Notes: The vertical bar in the figures indicates activity profile of defense-related enzymes from disease free (control) and after challenge to inoculation with M. oryzae (infected) leaf samples of 11 rice genotypes. The bar represent standard error of mean (n = 3) at p < 0.05 probability.

Figure 6. Activity of β-glycosidase.

Notes: The vertical bar in the figures indicates activity profile of defense-related enzymes from disease free (control) and after challenge to inoculation with M. oryzae (infected) leaf samples of 11 rice genotypes. The bar represent standard error of mean (n = 3) at p < 0.05 probability.

Figure 7. The purified genomic DNA was quantified and equal amount of DNA was used for PCR amplification. (a) Amplified genomic DNA of blast resistant and susceptible genotypes with SSR primer RM 224 and (b) with SSR RM144. The Lane M:100 bp, lane 1:1-KJT-2 (R),Lane 2:KJT-5 (R), Lane 3-TETEP (R), Lane 4:Chimansal(S), Lane 5:NLR-20104 (R),Lane 6:-EK-70 (S).

Jia, Y., & Moldenhauer, K. (2010). Development of monogenic and digenic rice lines for blast resistance genes. Journal of Plant Registrations, 4, 163–166.10.3198/jpr2009.04.0223crmp

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Anonymous. (2002). Find out how the qualities of rice are evaluated and scored in this authoritative sourcebook. Standard evaluation system for rice (p. 1518). Laguna: IRRI.

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