Antioxidant and antidiabetic potential of Myrothamnus flabellifolius found in Botswana

Figures & data

Table 1. Total phenol content of the Myrothamnus flabellifolius extracts

Leaf extract	TPC (mg GAE/g)
70% ethanol/water	350 ± 2.4
100% methanol	250 ± 2.4
100% chloroform	150 ± 1.6
70% methanol	300 ± 1.6
100% hexane	45 ± 1.8
Hexane	20 ± 0.2

Note: Values are the average of three trials ± standard deviation.

Figure 1. TLC-DPPH assay showing the anti-oxidant activities of different concentrations of MF spotted on a TLC sheet.

Figure 2. Comparative DPPH scavenging activity of Gallic acid and Myrothamnus flabellifolius.

Table 2. In vitro ABTS free radical scavenging activity of various extracts of Myrothamnus flabellifolius

Plant part	Test extract	IC50 (μg/ml)
Myrothamnus flabelifolious leaf and twig extract	100% hexane	77.21
	100% chloroform	66 .34
	100% methanol	55.13
	70% methanol/water	30.21
	70% Ethanol/water	25.22
Reference standard	Gallic acid	1.85

Notes: The values are mean of three replicates ± standard error. Extracts tested at 2.5, 5, 10, 25, 50 and 100 μg/ml. Reference standard (gallic acid) tested at 1, 2, 4, 8 and 16 μg/ml.

Figure 3. Comparative reducing power of Gallic acid and Myrothamnus flabellifolius leaf.

Figure 4. Comparative hydrogen peroxide scavenging activity of Gallic acid Myrothamnus flabellifolius extract.

Figure 5. Comparative nitric oxide scavenging activity of Gallic acid Myrothamnus flabellifolius extract.

Table 3. Phytochemical screening of Myrothamnus flabellifolius extracts

Compound	Methanol	70% methanol/water	Chloroform	70% ethanol/water	Hexane
Flavonoids	+	+	–	+	–
Tannins	+	+	–	+	–
Coumins	–	+	–	+	–
Terpenoids	–	+	+	+	–
Fatty acids	–	−	−	−	–
Phenols	–	+	−	+	–
Amino acids and proteins	–	–	–	–	–
Quinones	–	–	–	–	–
Oxalate	–	–	–	+	–
Triterpenoids	–	–	+	+	+
Saponins	–	+	–	+	–

Notes: + (present); − (Absent).

Figure 6. Percentage inhibition of Myrothamnus flabellifolius extracts on α-amylase. Each value is mean ± SEM of three trials.

Figure 7. Percentage inhibition of Myrothamnus flabellifolius extracts on α-Glucosidase. Each value is mean ± SEM of three trials.