β-amyloid infusion into lateral ventricle alters behavioral thermoregulation and attenuates acquired heat tolerance in rats

Figures & data

Figure 1. The mean T_ab in CN and Aβ–infused rats. (A) Before surgery, T_ab levels of CN (black circle) did not differ from those of Aβ-infused rats (red circle). (B) Twelve days after surgery, T_ab of the light phase in Aβ-infused rats was significantly higher than that of CN. Dark bars above abscissa indicate the dark phase of the day. Values are the means ±S.E.Ms (n = 6 in each group).

Figure 2. The mean T_s in CN and Aβ–infused rats. (A) T_s value of day in CN (black circle) and Aβ-infused rats (red circle). Dark bar above abscissa indicates the dark phase of the day. (B) Mean T_s of CN (black bar) and Aβ-infused rats (red bar) in the light and dark phase. In the light phase, mean T_s of Aβ-infused rats was significantly lower than that of CN. However, in the dark phase, mean T_s of Aβ-infused rats was significantly higher than that of CN. Values are the means±S.E.Ms (n = 6 in each group). *Shows significant difference of the light phase and dark phase. # shows significant difference between CN and Aβ-infused rats.

Figure 3. Heat tolerance test in CN and Aβ–infused rats. (A) T_a of the chamber in the heat tolerance test. T_a was gradually raised from time at 0 min. (B) T_ab responses to gradient heat of CN (black circle) and Aβ-infused rats (red circle). The T_ab values of all rats were monitored by a telemetry system. T_ab responses of CN and Aβ-infused rats did not significantly change. (C) c-Fos+ cells in the PO/AH. Green signals show c-Fos+ cells. The expression level of c-Fos+ cells of CN (left) and Aβ-infused rats (right) did not alter. Scale bar: 50 μm. (D) Over view of PO/AH of hypothalamic section. Red box shows immuno-stained area of (C). ac, anterior comm. ox, optic chiasm.

Figure 4. Ability of acquired heat tolerance in CN and Aβ-infused rats. (A) Heat tolerance test in CN (black circle) and Aβ–infused rats (red circle) after long term heat exposure. The increase in the T_ab values was slower for CN rats than that for the Aβ-infused rats. #, significant difference between CN and Aβ-infused rats. Values are the mean ±SEM (n = 4). (B) PCNA and (C) Dcx immunostaining in the hypothalamus. Scale bar, 100 µm. (D) Western blot analysis of Dcx protein in the hypothalamus. (Left) Western blot analyses were performed using anti-Dcx antibody. Blots were then stripped and reprobed with anti-GAPDH antibody to verify that equal amount of protein was electrophoresed in each lane. (Right) The densitometric data on Dcx/GAPDH of CN (black bar) and Aβ-infused rats (red bar). # shows significant difference between CN and Aβ-infused rats. Values are the mean ±SEM (n = 4).