Pre-treating dentin with chlorhexadine and CPP-ACP: self-etching and universal adhesive systems

Figures & data

Table 1. Instructions of use for the application of adhesive systems.

Adhesive system	Instructions of use
Clearfil SE Bond (Kuraray – Okayama, Japan)	Primer:
	1. Actively apply the primer on the surface for 20 s;
	2. Dry softly;
	Bond:
	1. Apply the adhesive on the surface;
	2. Dry softly with a jet of air;
	3. Photopolymerize for 10 s
Scotchbond Universal Adhesive (3M ESPE- Neuss, Germany)	1. Actively apply the adhesive on the surface for 20 s;
	2. Dry softly;
	3. Photopolymerize for 10 s

Table 2. Composition of the study materials.

Material	Composition
Clearfil SE Bond (Kuraray – Okayama, Japan)	1-Primer: water, MDP, HEMA, camphorquinone, hydrophilic dimethacrylate;
	2- Bond: MDP, Bis-GMA, camphorquinone, hydrophilic dimethacrylate, N, N-diethanol para-toluidine, colloidal silica
Scotchbond Universal Adhesive (3M ESPE- Neuss, Germany)	MDP, dimethacrylate resin, HEMA, polyalkenoic acid copolymer modified by methacrylate, load, silane, ethanol, water, primers
Filtek Z 350 XT (3 M ESPE/St Paul, MN-USA)	Ceramic treated with silane, Bis-GMA, Bis-EMA, silica treated with silane, zirconia-silica oxide treated with silane, TEG-DMA, BHT, pigments
MI Paste (GC Comp.- Japan)	Glirerol, CPP-ACP, D-sorbitol, propylene glycol, silica dioxide, titanium dioxide
Chlorhexidine 2% (VICOFARMA/Recife, PE- Brazil)	Chlorhexidine diglucanate 2%

Table 3. Division of the study groups.

Groups	Treatment
Group1	Clearfil SE Bond (Control)
Group 2	Clearfil SE Bond + MI Paste
Group 3	Clearfil SE Bond + Chlorhexidine 2%
Group 4	Clearfil SE Bond + Chlorhexidine 0.2%
Group 5	Scotchbond Universal Adhesive (control)
Group 6	Scotchbond Universal Adhesive + MI Paste
Group 7	Scotchbond Universal Adhesive + Chlorhexidine 2%
Group 8	Scotchbond Universal Adhesive + Chlorhexidine 0.2%

Table 4. Mean microtensile bond strength in mpa for the different adhesive systems and pretreatment of dentin.

Application technique	Clearfil SE bond	Scotchbond universal
Control (Manufacturer)	14.51 (3.14) ^(A,a)	15.86 (5.70) ^(A,a)
MI Paste	11.89 (2.34) ^(A,b)	15.22 (3.44) ^(A,a)
Chlorhexidine 2%	15.11 (5.42) ^(A,a)	16.36 (3.74) ^(A,a)
Chlorhexidine 0.2%	11.49 (3.70) ^(A,b)	20.25 (4.28) ^(A,a)

Different small letters indicate significant differences between the lines, based on the student’s t-test.

Different capital letters indicate significant differences between the columns, based on the F test (ANOVA).