Adding to the CASeload: unwarranted p53 signaling induced by Cas9

Figures & data

Figure 1. Implications of Cas9-induced p53 signaling. A summary of the biological, practical and clinical concerns associated with CRISPR associated protein 9 (Cas9)-induced tumor protein TP53 (p53) signaling. These concerns raise open questions that need to be tackled: from a basic science perspective, it will be important to understand the mechanisms that lead to nonspecific DNA cutting and to p53 signaling; from a routine lab work perspective, it will be helpful to determine best practices to monitor and alleviate Cas9-induced signaling; from a therapeutics perspective, it will be important to evaluate this issue in clinically-relevant settings. dCas9, catalytically-dead Cas9.