Impact of bacterial strain acquisition in the lung of patients with COPD: the AERIS study

Figures & data

Table 1. The number of patients and study timepoints for which sputum samples were positive for Haemophilus influenzae (NTHi) or Moraxella catarrhalis presence, acquisition, or apparition by culture, or acquisition or apparition of a strain identified by MLST (NTHi, sub-cohort; M. catarrhalis, full cohort).

	Number of visits with sputum available	Culture results			MLST results
		Number of bacteria-positive patients	Number of bacteria-positive visits	Number of visits with species acquisition/apparition	Number of patients with MLST data	Number of visits with MLST data	Number of visits with strain acquisition/apparition
NTHi (n = 36)*	Enrolment: 33	20	Enrolment: 9	NA	20	Enrolment: 10	NA
	Stable: 243		Stable: 73	6/15		Stable: 74	18/30
	Exacerbation: 113		Exacerbation: 45	3/12		Exacerbation: 45	13/21
M. catarrhalis (n = 122)*	Enrolment: 110	46	Enrolment: 8	NA	38	Enrolment: 7	NA
	Stable: 842		Stable: 42	16/31		Stable: 40	26/32
	Exacerbation: 320		Exacerbation: 40	21/32		Exacerbation: 26	19/22

n: number of patients; NA: not applicable; MLST: multilocus sequence typing.

The acquisition is defined as detection for the first time in the patient during the course of the study; apparition is defined as detection after a negative sputum sample from the patient at the preceding study visit (bacterial species or strain may have been detected at a visit before preceding visit; patient’s COPD status [stable or exacerbation] at preceding study visit not relevant).

*Number of patients with sputum sample available: 36 for NTHi; 122 (culture) and 121 (MLST) for M. catarrhalis.

Figure 1. Longitudinal analysis of non-typeable Haemophilus influenzae (NTHi) strain colonization in the sub-cohort and Moraxella catarrhalis strain colonization in the full cohort. Data is available from 20 NTHi-positive patients and 38 M. catarrhalis-positive patients. *Exacerbation. Strain changes in each patient indicated by successive changes in circle fill colour:

First strain detected;

Second strain detected;

Third strain detected;

Fourth strain detected;

Fifth strain detected.

Figure 2. Effect of non-typeable Haemophilus influenzae (NTHi) presence, acquisition, or apparition by culture, or acquisition or apparition of a strain identified by MLST on the odds of being in exacerbation vs. stable state (sub-cohort of 36 patients). Odds ratio results from case-control analyses performed on sputum culture results, using a logistic model stratified by subject, adjusted for the season. MLST: multilocus sequence typing; NS: not significant; 95% CI: 95% confidence interval.

Figure 3. Effect of Moraxella catarrhalis presence, acquisition, or apparition by culture, or acquisition or apparition of a strain identified by MLST on the odds of being in exacerbation vs. stable state (full cohort of 122 [culture] or 121 [MLST] patients). Odds ratio results from case-control analyses performed on sputum culture results, using a logistic model stratified by subject, adjusted for the season. MLST: multilocus sequence typing; 95% CI: 95% confidence interval.

Data availability statement

Anonymized individual subject data and study documents can be requested for further research from www.clinicalstudydatarequest.com.