T-RFLP analysis of soil bacterial structure from Cerrado within the Sete Cidades National Park, Brazil

Figures & data

Figure 1. Map presenting the gradient of cerrado at Sete Cidades National Park, Brazil.

Notes: ‘campo graminoide’ (CG), ‘cerrado strictu sensu’ (CSS) and ‘cerradao’ (CD).

Table 1. Main characteristics of the evaluated sites: ‘campo graminoide’ (CG), ‘cerrado strictu sensu’ (CSS) and ‘cerradao’ (CD).

Characteristic	CG	CSS	CD
Longitude	41°42′10″W	41°42′11″W	41°42″13″W
Latitude	04°07′21″S	04°07′22″S	04°7′28″S
Altitude (m)	215	221	238
Soil temperature (°C)	29	27	26
Vegetation	^a	^b	^c
Clay (g kg^−1)	210.2	235.8	251.4
Silt (g kg^−1)	100.7	121.3	104.5
Sand (g kg^−1)	689.1	642.9	644.1

^a Andropogon fastigiatus; Aristida longifólia; Eragrostis maypurensis.

^b Andropogon fastigiatus; Aristida longifolia; Terminalia fagifolia; Magonia pubescens; Hymenaea courbaril; Plathymenia reticulata; Qualea grandiflora; Combretum mellifluum; Lippia origanoides; Anacardium occidentale; Simarouba versicolor; Vatairea macrocarpa.

^c Aspidosperma discolor; Parkia platycephala; Terminalia fagifolia; Piptadenia moniliformis; Plathymenia reticulata; Qualea parviflora; Anacardium occidentale; Copaifera coriácea; Thiloa glaucocarpa; Casearia grandiflora.

Table 2. Average of soil chemical at different types of savannah: ‘campo graminoide’ (CG), ‘cerrado strictu sensu’ (CSS) and ‘cerradao’ (CD).

Site	TOC (g kg^−1)	pH	P (mg kg^−1)	K (cmolc kg^−1)	Al (cmolc kg^−1)	Ca (cmolc kg^−1)	Mg (cmolc kg^−1)	Na (cmolc kg^−1)
CG	4.3^b	4.9^a	3.5^a	1.4^a	0.23^a	0.10^a	0.02^b	0.48^a
CSS	8.2^a	4.7^a	4.6^a	1.8^a	0.36^a	0.33^a	0.11^a	0.49^a
CD	9.1^a	4.6^a	4.8^a	1.6^a	0.39^a	0.26^a	0.08^a	0.39^a

Table 3. Richness and diversity estimation of T-RFs derived from 16S rRNA based on enzymes Msp I and Hha I data, at different types of savannah: ‘campo graminoide’ (CG), ‘cerrado strictu sensu’ (CSS) and ‘cerradao’ (CD).

Site	Richness*	H′	S′	Whitaker**	Richness	H′	S′	Whitaker
	Hha I				Msp I
CG	119^b	4.16^a	0.53	0.77^b	157^b	4.31^a	0.46	0.98^b
CSS	124^a	4.01^a	0.52	1.00^a	162^a	4.32^a	0.48	1.05^a
CD	116^b	4.14^a	0.48	0.85^b	158^b	4.14^a	0.42	0.94^b

* Chao richness; H′ – Shannon index; S′ – evenness.

** Whitaker diversity.

Figure 2. Venn diagram of the operational taxonomic units (OTUs) based on the 16S rRNA gene with enzyme restriction: (a) Hha I enzyme and (b) Msp I enzyme.

Notes: ‘campo graminoide’ (CG), ‘cerrado strictu sensu’ (CSS) and ‘cerradao’ (CD).

Figure 3. Abundance of bacterial phyla derived from 16S rRNA based on enzymes Msp I and Hha I data, at different types of savannah: ‘campo graminoide’ (CG), ‘cerrado strictu sensu’ (CSS) and ‘cerradao’ (CD).

Figure 4. Non-metric multidimensional scaling (NMDS) plot of Principal Coordinates Analysis (PCoA), generated by Euclidean distance matrices, with 1000 permutations. (a) Structure Hha I and (b) structure Msp I, at different types of savannah: ‘campo graminoide’ (CG), ‘cerrado strictu sensu’ (CSS) and ‘cerradao’ (CD).

Figure 5. Distance-based redundancy analysis (db-RDA) of soil microbial communities. (a) Hha I enzyme and (b) Msp I enzyme.

Notes: Plots were generated using Bray-Curtis distance matrices with 1000 Monte-Carlo permutations. Vectors represent environmental variables, which were forward-selected (P_adjusted ≤ 0.05). Samples were colored as follows: ‘Campo graminoide’ (black circles), ‘Cerrado Strictu Sensu’ (gray circles) and ‘Cerradao’ (light gray circles).