Molecular characterization of mahogany tree (Swietenia macrophylla King, Meliaceae) in the remnant natural forest of Ecuador

Figures & data

Table 1. Location of the sites where S. macrophylla was sampled in Ecuador

Regions	Province	Samples	Geographical coordinates
Litoral Centro	Guayas (GY)	16	2°10′59″ S 80°00′57″ W
	Manabí (MN)	26	1°12′25″ S 80°22′15″ W
	Los Rios (LR)	19	1°11′02″ S 79°30′20″ W
Litoral Sur	El Oro (EO)	18	3°16′00″ S 79°58′00″ W
	Loja (LJ)	24	4°23′00″ S 79°57′00″ W
Amazonia	Napo (NP)	20	0°59′20″ S 77°48′57 ″W

Table 2. Estimates of genetic diversity in 123 S. macrophylla based on nine SSR loci according to Ref. [20]

Loci	Na	Ho	He	Fis
sm01	10	0.53	0.54	0.03
sm31	6	0.08	0.16	0.54
sm32	6	0.73	0.57	−0.28
sm34	6	0.28	0.43	0.34
sm40	7	0.31	0.36	0.15
sm45	7	0.68	0.53	−0.29
sm46	6	0.19	0.37	0.48
sm47	6	0.28	0.41	0.31
sm51	8	0.45	0.55	0.19
Average	6.89	0.39	0.43	0.16

N, samples number; Na, observed number of alleles; Ho, observed heterozygosity; He, expected heterozygosity; Fis, inbreeding coefficient.

Table 3. Descriptive statistics overall loci for each population according to Ref. [20]

Population	N	NA	PA	Ho	He	Fis
Guayas (GY)	16	4.44	4	0.60	0.64	0.034
Manabí (MN)	26	5.78	5	0.24	0.63	0.66
Los Ríos (LR)	19	1.33	0	0.33	0.17	−0.98
El Oro (EO)	18	1.33	0	0.33	0.17	−0.98
Loja (LJ)	24	3	1	0.44	0.56	0.20
Napo (NP)	20	2.22	4	0.39	0.46	0.12
Average	123	3.02	2.33	0.39	0.44	0.07

N: samples number, Na: observed number of alleles by population, PA: privative alleles. Ho: observed heterozygosity, He: expected heterozygosity.

Table 4. Analyses of Molecular Variance (AMOVA) in six provinces of Ecuador, located on farm and natural populations of S. macrophylla.

Sources of variation	Degrees of freedom	Sum of square	Mean square	Est. variance	Percentage variation
Among Population	5	76.5	15.3	0.892	8
Within Population	117	1134.9	9.7	9.681	92
Total	122	1211.4		10.573

Figure 1. Genetic cluster identified by Bayesian analysis using Structure and Harvester software. (a) ∆K estimates of the posterior probability distribution of the data for a given K. Ln (K) obtained are a Structure analysis and an ad hoc quantity distribution. (b) Estimated population structure and the genetic clusters of the six provinces of Ecuador and 123 individuals of S. macrophylla. Guayas (GY), Manabí (MN), Los Ríos (LR), El Oro (EO), Loja (LJ) and Napo (NP).

Peakall R, Smouse PE. GENALEX 6: genetic analysis 364 in excel. Population genetic software for teaching and research. Mol Ecol Notes. 2006;6(1):288–295.

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