Amplification of mtDNA control region in opportunistically collected bird samples belonging to nine families of the order Passeriformes

Figures & data

Table 1. Primer sequences for the amplification of control region of bird species and expected length of their amplicons.

S. No.	Primer name/combination	Sequence (5′-3′)/Expected amplicon length
1	FLCF1	GAA TGG GGT CAA AGT GCA TCA GT
2	FLCF2	TGA TGG ACA TGT CAA GAG GAA G
3	FLCF3	GG CGC AAA AGA GCA AGT
4	FLCR1	ACT TGC TCT TTT GCG CC
5	FLCF4	GTA GCT CGG TTC TCG TGA GAA
6	FLCR2	TTC TCA CGA GAA CCG AGC TAC
8	FLCR3	CCT GAA AAG CCG CTG TTA T
9	FLCR4	TCC ATC TCC AGC TCC CAA AGC
Primer combination and expected length
i.	FLCF1+ FLCR1	320 (bp)
ii.	FLCF1 + FLCR2	410 (bp)
iii.	FLCF2+ FLCR1	217 (bp)
iv.	FLCF2+ FLCR2	300 (bp)
v.	FLCF4+ FLCR3	510 (bp)
vi.	FLCF3+ FLCR4	569 (bp)

Figure 1. Gel image showing the amplification result of mtDNA control region from the DNA extracted from bird samples using primer FLCF1 + FLCR1 (lane 1); FLCF1 + FLCR2 (lane 2); FLCF2 + FLCR1 (lane 3); FLCF2 + FLCR2 (lane 4); FLCF4 + FLCR3 (lane 5); FLCF3 + FLCR4 (lane 6).