Complete mitochondrial genome of mouthbrooding fighting fish (Betta pi) compared with bubble nesting fighting fish (B. splendens)

Figures & data

Figure 1. Phylogenetic relationships among concatenated mitochondrial twelve protein-coding genes without ND6 sequences of 19 mitochondrial genomes including Eleotris acanthopoma as the outgroup inferred using Bayesian inference analysis. The complete mitochondrial genome sequence was downloaded from GenBank. The accession number is indicated in parentheses after the scientific name of each species. Support values at each node are Bayesian posterior probabilities. The branch-lengths represent number of nucleotide substitutions per site.

Table 1. Comparison of nucleotide length, AT content, start codon, stop codon and nucleotide sequence diversity between the three complete mitochondrial genomes of Betta pi (AB920288), B. splendens (AB571120) and B. splendens (KR527219).

	Sequence length (bp)			AT content (%)			Start codon			Stop codon			Nucleotide sequence diversity (%)^b
Gene	BPI	BSP1	BSP2	BPI	BSP1	BSP2	BPI	BSP1	BSP2	BPI	BSP1	BSP2	BPI/BSP1 (%)	BPI/BSP2 (%)	BSP1/BSP2 (%)
12S rRNA	955	956	960	56.75	56.59	58.84	–	–	–	–	–	–	13.24	13.45	2.01
16S rRNA	1676	1701	1690	57.58	59.53	59.47	–	–	–	–	–	–	13.50	13.70	0.30
ND1	975	975	975	58.26	61.64	61.95	ATG	ATT	ATT	TAA	TAA	TAA	23.79	21.23	3.18
ND2	1046	1046	1046	57.84	61.00	61.00	ATG	ATG	ATG	TA+^a	TA+ ^a	TA+ ^a	23.33	23.14	0.19
COI	1560	1560	1560	54.81	59.94	59.94	GTG	GTG	GTG	AGG	AGG	AGG	19.94	19.74	0.32
COII	691	691	691	58.76	61.22	61.08	ATG	ATG	ATG	T++^a	T++^a	T++^a	22.43	22.43	0.14
ATP8	168	168	168	60.12	69.64	69.65	ATG	GTG	GTG	TAA	TAA	TAA	30.95	30.95	0.00
ATP6	683	684	702	57.25	65.45	64.96	ATG	ATG	ATG	TA+^a	TA+^a	TAA	22.25	22.40	0.59
COIII	786	798	786	54.20	59.65	59.41	ATG	ATG	ATG	TAA	TAA	TAA	18.58	18.70	0.13
ND3	349	349	349	56.73	59.03	59.03	ATG	ATG	ATG	T++^a	T++^a	T++^a	21.84	20.98	0.86
ND4L	297	297	297	55.22	57.58	57.58	ATG	ATG	ATG	TAA	TAA	TAA	20.88	20.88	0.00
ND4	1381	1381	1381	57.86	62.42	62.64	ATG	ATG	ATG	T++^a	T++^a	T++^a	22.30	22.23	0.29
ND5	1846	1851	1863	59.15	63.10	63.12	ATG	ATA	GTG	T++^a	TAA	TAA	29.27	29.38	0.11
ND6	522	522	522	56.90	63.10	63.03	ATG	ATG	ATG	TAA	TAA	T++^a	24.76	24.76	0.00
CytB	1156	1147	1147	56.84	62.12	62.34	ATG	ATG	ATG	T++^a	T++^a	T++^a	22.67	22.84	0.35
Control region	847	1199	1327	64.58	74.71	77.62	–	–	–	–	–	–	22.54	22.54	0.24
Entire genome	16521	16980	17099	57.72	61.91	62.13							20.61	20.49	0.64

^a TAA stop codon is completed by the addition of 3’A residues to mRNA.

^b Betta pi: BPI; B. splendens (AB571120): BSP1; and B. splendens (KR527219): BSP2.