A rapid and efficient method for enriching mitochondrial DNA from plants

Figures & data

Table 1. Primer pairs used for real-time quantitative PCR of nuclear, mitochondrial and plastid DNA, along with annealing temperature and amplicon size for each pair.

Compartment	Gene, accession	Primers (5′–3′)	Tm (°C)	Amplicon size
Nucleus	Leafy, AF466801.1	CAACGAAGGTGAGGATGACG GATAAACGGATGCTCCCTCTG	60	88 bp
Plastid	matK, AP000423.1	TCACGGAAGATGCATTCTGG TGACCTTTTGCGATTGAAACC	60	119 bp
Mitochondrion	nad9, JF729201.1	AGTAAGTCTATTTCCATCAGCCG CACTCAGAGGAAGGTCTTTTCG	60	149 bp

Table 2. Results of qPCR analysis. Averages and standard deviations of Cq values of the biological replicates for the two species and two methods used are shown for the primer pairs from each cellular compartment.

	Cq values, avg ± SD			Copy number relative to nucleus
Species and method	Mitochondria	Chloroplast	Nucleus	Mitochondria	Chloroplast
A. thaliana DNeasy	21.90 ± 0.26	17.39 ± 0.29	26.27 ± 0.36	20.9 ± 3.06	477 ± 58
A. thaliana MitoPrep	23.85 ± 0.37	21.96 ± 0.49	33.00 ± 0.16	604 ± 214*	2171 ± 514*
B. rapa DNeasy	19.26 ± 0.12	27.07 ± 0.94	34.31 ± 0.96	42391 ± 32294	314 ± 359
B. rapa MitoPrep	23.96 ± 0.19	29.88 ± 0.24	N/A	1350963 (est.)*	25126 (est.)

* p < .05.