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Mito Communication

Forensically informative nucleotide sequencing (FINS) for species and subspecies of genus Prionailurus (Mammalia: Carnivora: Felidae) through mitochondrial genes (12SrRNA and cytochrome b) by using old taxidermy samples

Pages 615-619 | Received 14 Mar 2018, Accepted 03 Apr 2018, Published online: 17 May 2018

Figures & data

Table 1. Sample details.

Figure 1. 12SrRNA based ML tree topology (A) and NJ tree topology (B) of species and subspecies of Prionailurus. The evolutionary distances were computed using the Kimura two-parameter method and are in the units of the number of base substitutions per site. The analysis involved seven nucleotide sequences including outgroup Canis lupus lupus. Median-joining network by using 12SrRNA gene of genus Prionailurus indicating four haplotypes of species and subspecies of Prionailurus. Haplotype 5 belongs to outgroup (C).

Figure 1. 12SrRNA based ML tree topology (A) and NJ tree topology (B) of species and subspecies of Prionailurus. The evolutionary distances were computed using the Kimura two-parameter method and are in the units of the number of base substitutions per site. The analysis involved seven nucleotide sequences including outgroup Canis lupus lupus. Median-joining network by using 12SrRNA gene of genus Prionailurus indicating four haplotypes of species and subspecies of Prionailurus. Haplotype 5 belongs to outgroup (C).

Figure 2. Cytochrome b based ML tree topology (A) and NJ tree topology (B) of species and subspecies of Prionailurus. The evolutionary distances were computed using the Kimura two-parameter method and are in the units of the number of base substitutions per site. The analysis involved seven nucleotide sequences. Median-joining network (C) by using cytochrome b gene of genus Prionailurus indicating six haplotypes of species and subspecies of Prionailurus. Haplotype 7 belongs to outgroup.

Figure 2. Cytochrome b based ML tree topology (A) and NJ tree topology (B) of species and subspecies of Prionailurus. The evolutionary distances were computed using the Kimura two-parameter method and are in the units of the number of base substitutions per site. The analysis involved seven nucleotide sequences. Median-joining network (C) by using cytochrome b gene of genus Prionailurus indicating six haplotypes of species and subspecies of Prionailurus. Haplotype 7 belongs to outgroup.

Table 2. Observed DNA diversity in 12Sr RNA and cytochrome b gene.