Population genetic diversity of an odorous frog Odorrana grahami (Amphibia: Anura: Ranidae) in relation to conservation based on mitochondrial DNA

Figures & data

Figure 1 Sampling localities in this study and phylogenetic relationships of Odorrana grahami. (A) Sampling localities (P1–P10) in this study. Inset photo shows O. grahami. (B) Maximum likelihood tree based on COI gene sequences of haplotypes of O. grahami. Bootstrap supports with >50% values are denoted near nodes. (C) Haplotype network of O. grahami. The circle size is proportional to the number of samples. One white dot means one mutation.

Table 1. Genetic diversity and neutrality tests of O. grahami populations.

Population ID	Locality	Latitude	Longitude	n	h	π	Tajima’s D	Fu’s Fs
P1	Liupanshui City, Guizhou Province	26.6859°	104.6316°	3	0.000	0.00000
P2	Weining County, Guizhou Province	26.8503°	104.2020°	1	0.000	0.00000
P3	Huili County, Sichuan Province	26.7766°	102.2724°	18	0.608	0.00228	−0.24781	0.560
P4	Jinyang County, Sichuan Province	27.7258°	103.2743°	1	0.000	0.00000
P5	Mianning County, Sichuan Province	28.9324°	102.2390°	30	0.405	0.00099	−0.53872	−0.858
P6	Yanyuan County, Sichuan Province	27.9936°	100.9901°	3	0.000	0.00000	−	−
P7	Dali City, Yunnan Province	25.8495°	100.0956°	7	0.286	0.00049	−1.00623	−0.095
P8	Kunming City, Yunnan Province	24.9658°	102.6075°	9	0.000	0.00000
P9	Shuangbai County, Yunnan Province	24.2760°	101.7859°	2	0.000	0.00000
P10	Yimen County, Yunnan Province	23.9649°	102.5524°	2	1.000	0.00170
Total	–	–	–	76	0.605	0.00199	−0.71828	−1.788*

n: number of samples; h: haplotype diversity; π: nucleotide diversity.

Significance level: *p < .05.

Table 2. Distribution and number of individuals of the eight haplotypes in ten population of O. grahami.

Population ID	Haplotype
	H1	H2	H3	Η4	H5	H6	H7	H8
P1	3	–	–	–	–	–	–	–
P2	–	–	–	–	–	1	–	–
P3	6	1	–	–	–	10	1	–
P4	1	–	–	–	–	–	–	–
P5	3	–	–	–	3	23	–	1
P6	3	–	–	–	–	–	–	–
P7	6	–	1	–	–	–	–	–
P8	9	–	–	–	–	–	–	–
P9	2	–	–	–	–	–	–	–
P10	1	–	–	1	–	–	–	–