Utility of mitochondrial DNA in wildlife forensic science: reliable identification of confiscated materials from Eastern India

Figures & data

Table 1. Details of the confiscated wildlife materials received at ZSI, Kolkata for species identification.

Sample IDs	DNA source	Gene	Accession Number	Species identification	Common Name	Conservation status
						IUCN	CITES	IWPA
ZSI_MAM-5	Raw meat	COI/Cytb	KF808255/MH117926	Bos gaurus	Indian bison	Vulnerable	Appendix I	Schedule I
ZSI_MAM-14	Cooked meat	Cytb	MH117919	Bos indicus	Humped cattle	Not Accessed	Not Accessed	Not Accessed
ZSI_MAM-1	Raw meat	Cytb	MH117927	Sus scrofa	Wild boar	Least Concern	Not Accessed	Schedule III
ZSI_MAM-11	Cooked meat	Cytb	MH117921
ZSI_MAM-13	Cooked meat	Cytb	MH117920
ZSI_MAM-15	Raw meat	Cytb	MH117918
ZSI_MAM-22A	Cooked meat	Cytb	MH117915
ZSI_MAM-21C	Dried Skin	Cytb	MH117914	Muntiacus muntjak	Barking deer	Least Concern	Not Accessed	Schedule III
ZSI_MAM-22B	Raw meat	Cytb	MH117916	Garrulax leucolophus	White-crested laughingthrush	Least Concern	Not Accessed	Not Accessed
ZSI_MAM-23	Dried Skin	Cytb	MH117917	Neofelis nebulosa	Clouded leopard	Vulnerable	Appendix I	Schedule I
ZSI_MAM-25A	Raw meat	Cytb	MH117930	Rhinoceros unicornis	Greater one-horned rhinoceros	Vulnerable	Appendix I	Schedule I
ZSI_MAM-25B	Raw meat	Cytb	MH117931
ZSI_MAM-25C	Raw meat	Cytb	MH117932
ZSI_MAM-25D	Raw meat	Cytb	MH117933
ZSI_MAM-26	Dried Skin	Cytb	MH117929	Axis axis	Chital	Least Concern	Not Accessed	Schedule III
ZSI_MAM-27A	Raw meat	Cytb	MH117924
ZSI_MAM-27B	Raw meat	Cytb	MH117925
ZSI_REP-16	Semi-liquid venom	Cytb	MH117922	Naja naja	Indian cobra	Not Accessed	Appendix II	Schedule II
ZSI_REP-17	Semi-liquid venom	Cytb	MH117923
ZSI_REP-18	Raw meat	Cytb	MH117928	Lepidochelys olivacea	Olive ridley turtle	Vulnerable	Appendix I	Schedule I
ZSI_REP-19	Cloacal Swab	COI	MH117913	Python molurus	Indian rock python	Vulnerable	Appendix I	Schedule I

WB: West Bengal; COI: Cytochrome C oxidase Subunit I; Cytb: Cytochrome b; IUCN: International Union for Conservation of Nature; CITES: Convention on International Trade in Endangered Species of Wild Fauna and Flora; IWPA: Indian Wildlife Protection Act.

Figure 1. The neighbour-joining trees of the studied confiscated wildlife materials for clustering based species identification. (A) Muntiacus muntjak (B) Bos gaurus (C) Lepidochelys olivacea (D) Python molurus (E) Sus scrofa (F) Naja naja (G) Garrulax leucolophus (H) Rhinoceros unicornis (I) Axis axis (J) Neofelis nebulosa. The generated sequences were marked by black dots in the trees and depicts the cohesive clustering with the available reference sequences acquired from GenBank database. The trees also represent the relationship of the examined species with other congeners.