Single nucleotide polymorphisms in the D-loop region predicts earlyage-at-onset of malignant fibrous histiocytoma

Figures & data

Figure 1. Comparison of age-at-onset for MFH patients according to the genotype of nucleotides 16304 in the mitochondrial D-loop with the Kaplan–Meier method.

Figure 2. Comparison of age-at-onset for MFH patients according to the genotype of nucleotide AC523-524del in the mitochondrial D-loop with the Kaplan–Meier method.

Table 1. Clinical characteristics and their association with age-at-onset age in MFH patients.

Characteristics	No. of cases	p Value
Metastasis	39	.056
Relapse	22	.509
Location		<.001
Extremities	35
Head and neck	10
Retroperitoneum and abdominal	8
Trunk wall	27
Pathological		.395
Mucinous	11
Storiform	62
Giant cell	6
Inflammatory	1

Table 2. The 27 SNP sites with a rare allele frequency higher than 5% and their associations with metastasis and relapse in MFH patients by Kaplan–Meier method.

			Metastasis			Relapse
SNP sites		No. of cases	Yes	No	p Value	Yes	No	p Value
C16261T	C	76	20	56	.304	37	39	.959
	T	4	2	2		2	2
C16266T	C	74	21	53	.539	39	35	.014
	T	6	1	5		0	6
C16290T	C	69	20	49	.459	32	37	.291
	T	11	2	9		7	4
T16298C	T	76	21	55	.909	37	39	.959
	C	4	1	3		2	2
T16304C	T	71	19	52	.679	35	36	.785
	C	9	3	6		4	5
T16311C	T	72	19	53	.507	34	38	.415
	C	8	3	5		5	3
G16319A	G	66	20	46	.226	29	37	.063
	A	14	2	12		10	4
T16362C	T	50	12	38	.368	22	28	.276
	C	30	10	20		17	13
T16519C	T	47	11	36	.331	23	24	.968
	C	33	11	22		16	17
C63T	C	72	20	52	.868	35	37	.941
	T	8	2	6		4	4
A72G	A	3	0	3	.280	0	3	.087
	G	77	22	55		39	38
C113T	C	76	21	55	.909	37	39	.959
	T	4	1	3		2	2
T146C	T	71	20	51	.708	34	37	.667
	C	9	2	9		5	4
C150T	C	75	22	53	.158	38	47	.187
	T	5	0	5		1	4
C151T	C	69	19	50	.986	35	34	.378
	T	11	3	8		4	7
T152C	T	76	18	58	.001	36	40	.284
	C	4	4	0		3	1
A176del	A	69	20	49	.459	33	36	.681
	del	11	2	9		6	5
T195C	T	71	20	51	.708	35	36	.785
	C	9	2	7		4	5
A235G	A	67	20	47	.288	33	34	.839
	G	13	2	11		6	7
A249del	A	61	17	44	.895	32	29	.237
	del	19	5	14		7	12
A263G	A	6	2	4	.741	4	2	.364
	G	74	20	54		35	39
T310CTC	T	49	15		.436	26	23	.335
	CTC	31	7	24		13	18
311-312 TCCinsert		76	20	56	.304	37	33	.959
	TCC	4	2	2		2	2
315-316 Cinsert		40	11	29	1.00	19	21	.824
	C	40	11	29		20	20
G366A	G	76	21	55	.909	36	40	.284
	A	4	1	3		3	1
T489C	T	47	13	34	.970	23	24	.968
	C	33	9	24		16	17
AC523-524del	A	58	14	44	.277	28	30	.891
	del	22	8	14		11	11

Figure 3. Comparison of age-at-onset for MFH patients according to the tumor location by the Kaplan–Meier method.

Table 3. Correlation of metastasis and relapse with other clinical characteristics in MFH patients.

	Metastasis			Relapse
Variable	Yes	No	p	Yes	No	p
Number	22	58		39	41
Age	58(10–87)	63(39–79)	.140	52(10–87)	64(32–86)	.019
Gender			.197			.076
Male	16	33		20	29
Female	6	25		19	12
Location			.197			.014
Extremities	5	29		23	11
Head and neck	4	5		2	7
Retroperitoneum and abdominal	5	2		3	4
Trunk wall	8	22		11	19
Pathological type			.297			.974
Mucinous	5	6		3	8
Storiform	15	47		35	27
Giant cell	2	4		1	5
Inflammatory	0	1		0	1