The genetic variations in the mitochondrial genomes of three Luciolinae fireflies

Figures & data

Table 1. Specimen collection and storage information.

Firefly species	Number	Collection information	Storage site of samples/reference
Pygoluciola qingyu	F9	Niujie township, Yiliang County, Yunnan Province	Chen et al. 2019
Abscondita terminalis	F11	Menglun township, Mengla County, Xishuangbanna Prefecture, Yunnan Province
Emeia pseudosauteri	F15	Tiantaishan, Qionglai city, Sichuan Province
Pygoluciola qingyu	F10	Jiugong Mountain, Tongshan County, Xianning City, Hubei Province	Natural History Museum, Huazhong Agricultural University, Wuhan City, Hubei Province, China
Abscondita terminalis	F12	Jiangxia District, Wuhan city, Hubei Province
Emeia pseudosauteri	F16-A	Xiapu town, Tongshan County, Xianning city, Hubei Province
Emeia pseudosauteri	F16-B	Lv Xin Garden, Leshan city, Sichuan Province
Emeia pseudosauteri	F16-C	Gaojiayang town, Changyang County, Yichan city, Hubei Province
Vesta saturnalis	F22	China, Yunnan: Xishuangbanna Prefecture, Mengla County, Menglun Township, Tropical Botanic Garden	Chen et al. 2019

Table2. The COI sequences of 24 species were obtained from the GenBank database.

Number	Subfamily	Species	Accession No.	Cite References
F1	Luciolinae	Luciola substriata	KP313820	Mu et al. 2015
F2		Asymmetricata circumdata	KX229747	Luan and Fu 2016
F3		Pteroptyx maipo	MF686051	Fan and Fu 2017
F4		Aquatica leii	KF667531	Jiao et al. 2015
F5		Aquatica ficta	KX758085	Wang et al. 2017
F6		Aquatica wuhana	KX758086	Wang et al. 2017
F7		Aquatica lateralis	LC306678	Maeda et al. 2017
F8		Luciola cruciata	AB849456	Amano et al 2013
F9		Pygoluciola qingyu	MK292093	Chen et al. 2019
F10		Pygoluciola qingyu	In this study	Tongshan County
F11		Abscondita terminalis	MK292092	Chen et al. 2019
F12		Abscondita terminalis	In this study	Wuhan city
F13		Abscondita chinensis	MK122952	Wang and Fu 2019
F14		Abscondita anceyi	MH020192	Hu and Fu 2018
F15		Emeia pseudosauteri	MK292112	Chen et al. 2019
F16-A		Emeia pseudosauteri	In this study	Tongshan County
F16-B		Emeia pseudosauteri	In this study	Leshan City
F16-C		Emeia pseudosauteri	In this study	Changyang County
F17		Curtos costipennis	MK609965	Zhang and Fu 2019
F18	Lampyrinae	Pyrocoelia rufa	AF452048	Bae et al 2004
F19		Diaphanes citrinus	MK292103	Yang and Fu 2019
F20	Tribolium	Tribolium castaneum	NC003081	Friedrich and Muqim 2003
F21	Chauliognathus	Chauliognathus opacus	FJ613418	Sheffield et al. 2009
F22	Amydetinae	Vesta saturnalis	MK292111	Chen et al. 2019

Table 3. Basic description of the complete mitochondrial genomes of the three species.

Species	Pygoluciola qingyu (F10)	Abscondita terminalis (F12)	Emeia pseudosauteri (F16)
Base length	17,332 bp	16,398 bp	16,956 bp
Base composition
A	45.59%	41.19%	44.21%
C	11.66%	11.95%	11.46%
G	7.96%	8.24%	8.32%
T	34.71%	35.62%	36.00%
Initiation codon
ATT	COI, D5, ND3, ATP8	COI, ND3, ND4L	COI
ATA	ND6, ND2	ND2, ATP8, ND5, ND6, ND1	ND2, ND5, ND3, ND6, ATP8
ATG	COII, OIII, ND4, ND4L, ATP6, CTYB	COII, COIII, ND4, ATP6, CTYB	COII, COIII, ND4, ND4 L, ATP6, CTYB, ND1
TTG	ND1
Terminal codon
Single T	COII, ND5, ND4, COI	COII, COIII, ND5, ND4, COI	COII, COIII, ND5, ND4, COI
TAA	ND2, ND6, ATP8, ATP6, ND4L	ND2, ND6, ATP8, ATP6, ND4L, D4L	ND2, ND6, ATP8, ATP6, ND4L
TAG	ND1, ND3, CTYB	ND1, CTYB	ND1, ND3, CTYB
AGA	COIII		COIII
GenBank accession No.	MN688374	MN722653	MN722654

Figure 1. Molecular phylogenetic analysis by maximum likelihood method.

Table 4. Estimates of evolutionary divergence between 18sDNA sequences.

Species	1	2	3	4	5
Emeia pseadosauteri in this study F16-A
Emeia pseadosauteri in this study F16-B	0.000
Emeia pseadosauteri in this study F16-C	0.000	0.000
Vesta saturnalis MK292083 F22	0.000	0.000	0.000
Emeia pseudosauteri MK292085 F15	0.582	0.582	0.582	0.582

Table 5. Estimates of evolutionary divergence between MtDNA sequences.

Species	1	2	3
Emeia pseadosauteri in this study F16-A
Vesta saturnalis MK292083 F22	0.022
Emeia pseudosauteri MK292085 F15	0.234	0.234

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