A new set of highly efficient primers for COI amplification in rotifers

Figures & data

Table 1. Sequences information of three primers used in this study.

Primer	Nucleotide sequence (5’-3’)	GC (%)	Tm (°C)	Size (bp)
30F	F:HACTAATCAYAARGATATTGGWAC	25.0	51	850
885R	R:RAACATATGATGAGCYCAWACAAT	29.2
LCO2198	F:GGTCAACAAATCATAAAGATATTGG	32.0	51	700
HCO1490	R:TAAACTTCAGGGTGACCAAAAAATCA	34.6
dgLCO	F:GGTCAACAAATCATAAAGAYATYGG	32.0	47	700
dgHCO	R:TAAACTTCAGGGTGACCAAARAAYCA	34.6

Figure 1. Electrophoretogram of PCR amplification for 22 rotifers: (1) K. cochlearis, (2) K. tropica, (3) K. tecta, (4) B. diversicornis, (5) B. falcatus, (6) B. caudatus, (7) B. urceolaris, (8) B. angularis, (9) B. quadridentatus, (10) B. forficula, (11) A. priodonta, (12) A. brightwelli, (13) P. vulgaris, (14) T. dixonnuttalli, (15) T. capucina, (16) T. cylindrica, (17) S. oblonga, (18) L. bulla, (19) Lecane sp1., (20) Lecane sp2., (21) A. ovalis, (22) P. hudsoni.

Figure 2. PCR success rates of three primers in nine genera rotifers (Keratella, Brachionus, Asplanchna, Polyarthra, Trichocera, Synchaeta, Lecane, Ascomorpha, Ploesoma).

Figure 3. The positions of primers of different rotifers on the COI.

Data availability statement

Newly sequenced COI sequence in this study is openly available in NCBI with GenBank number: MT908928 to MT908946.

Diamond reference library, 9 partial mitochondrial genomes of rotifera and supplementary information can be found at https://github.com/ShaolinXU/New-COX1-primers-for-rotifera.