The complete mitochondrial genome of De Brazza’s monkey (Cercopithecus neglectus)

Figures & data

Table 1. Sequences of primers used in amplification of the complete mitochondrial genome in C. neglects.

Number	Primer sequences (5’-3’)	Gene region
1	1-F 5′-GCAAGACACTGAAGATGCCTAGA-3′ 1-R 5′-CGTGCTCGGGTGATTTTATT-3′	25–1236 = 1212
2	2-F 5′-GACCTACACTCGGAAGATCTCAATA-3′ 2-R 5′-TGCTTAGGCTTGTGCAGTCTTAG-3′	1047–2293 = 1247
3	3-F 5′-ATTGACCTGTCCGTGAAGAGA-3′ 3-R 5′-AGCTATGAAAAATAAGGCGAATG-3′	2092–3402 = 1311
4	4-F 5′-CGGCTCATTTAACCTTAACATACTC-3′ 4-R 5′-GTAATGGTTGGGTTATATGCTAGTG-3′	3195–4488 = 1294
5	5-F 5′-AAAACTAGCCCCCATCTCAATTA-3′ 5-R 5′-AGCCAGAAGCTTATGTTGTTTAGAC-3′	4288–5630 = 1343
6	6-F 5′-GTCAACCCGGTAGCTTATTAGG-3′ 6-R 5′-ATCACTGCTACTAACGAAATAAAGG-3′	5444–6716 = 1273
7	7-F 5′-CAAAGCTCACTTCATCGTTATATTC-3′ 7-R 5′-TAGCTGTGCAGTGCGCTTGTA-3′	6549–7721 = 1123
8	8-F 5′-AAACTACATTCACCGCCATACG-3′ 8-R 5′-AGATGTATTAGTAGGTGACCAGCTG-3′	7543–8454 = 912
9	9-F 5′-CATTTACACCTACCACCCAACTATC-3′ 9-R 5′-GTTGGGGTAATCAGAGTATAATGGT-3′	8208–9547 = 1340
10	10-F 5′-GCCGCTTGATACTGACACTTTG-3′ 10-R 5′-GGGATGAGAGTGGTTTCAAATAAG-3′	9328–10,552 = 1225
11	11-F 5′-CCAGCCAATATCACCTATACAATG-3′ 11-R 5′-CTAAGGCCAATGGATAGCTGTT-3′	10,405–11,710 = 1306
12	12-F 5′-CTCACCCCAATCATCCTTCTATC-3′ 12-R 5′-GTTGATGCCGATGGTGACTAT-3′	11,490–12,703 = 1214
13	13-F 5′-GGTGTTCCTACTCATTCGCTTTC-3′ 13-R 5′-TTTTTGGTTATACTACGGCGATG-3′ 13-FC 5′-AACCAACAATGCCCTACTACCTA-3′	12,511–13,878 = 1368 13,179
14	14-F 5′-CTCAAAAATTATCCAGCTCTCTATG-3′ 14-R 5′-TGCAAATAGGAAGTATCACTCTGGT-3′	13,697–14,978 = 1282
15	15-F 5′-AATCACTTTTCACCCCTACTACACA-3′ 15-R 5′-CGTGCGGACCAGAGATAAAAG-3′	14,798–15,900 = 1102
16	16-F 5′-AACTGTATCCGACATCTGGTTCTTA-3′ 16-R 5′-GTGTGGCTGTGCAAAGTGTT-3′	15,738–223

Table 2. The composition of the mitogenome of C. neglectus.

Gene	Position		Size	Codons		Anti-condon	Strand	Space/Overlap
	Start	End		Start	End
tRNA-Phe	1	70	70			GAA	+	0
rrnS	135	377	949				+	0
tRNA-Val	1020	1088	69			TAC	+	0
rrnL	1089	2647	1559				+	0
tRNA-Leu	2648	2722	75			TAA	+	2
nad1	2725	3679	955	ATG	TAG		+	0
tRNA-Ile	3680	3748	69			GAT	+	−3
tRNA-Gln	3746	3817	72			TTG	−	1
tRNA-Met	3819	3886	68			CAT	+	0
nad2	3887	4928	1042	ATC	TAG		+	0
tRNA-Trp	4929	4994	66			TCA	+	7
tRNA-Ala	5002	5069	68			TGC	−	1
tRNA-Asn	5071	5143	73			GTT	−	32
Trna-Cys	5176	5242	67			GCA	−	−1
Trna-Tyr	5242	5307	66			GTA	−	12
cox1	5320	6888	1569	ATG	TAA		+	−28
Trna-Ser	6861	6929	69			TGA	−	3
Trna-Asp	6933	7000	68			GTC	+	1
cox2	7002	7685	684	ATG	TAA		+	23
Trna-Lys	7709	7774	66			TTT	+	2
atp8	7777	7977	201	ATG	TAA		+	−41
atp6	7937	8617	681	ATG	TAA		+	−1
cox3	8617	9400	784	ATG	TA(A)		+	0
Trna-Gly	9401	9468	68			TCC	+	0
nad3	9469	9812	344	ATT	T(AA)		+	2
Trna-Arg	9815	9879	65			TCG	+	0
nad4l	9880	10,176	297	ATG	TAA		+	−7
nad4	10,170	11,547	1378	ATG	T(AA)		+	0
Trna-His	11,548	11,616	69			GTG	+	0
Trna–Ser	11,617	11,675	59			GCT	+	0
Trna-Leu	11,676	11,746	71			TAG	+	6
nad5	11,753	13,555	1803	ATA	TAA		+	0
nad6	13,556	14,074	519	ATG	AGG		−	0
Trna-Glu	14,075	14,143	69			TTC	−	4
cob	14,148	15,288	1141	ATG	T(AA)		+	0
Trna-Thr	15,289	15,356	68			GTA	+	2
Trna-Pro	15,359	15,426	68			TGG	−	375
D-loop	15,802	15,981	180				+	509

Figure 1. Phylogenetic relationship of 13 primate species based on Maximum likelihood model. Genbank accession Number: Homo sapiens (DQ246832.1), Gorilla gorilla (NC001645.1), Hylobates moloch (CM020645.1), Varecia variegate variegate (NC012773.1), Presbytis melalophos mitrata (KY117601.1), Rhinopithecus bieti (HM125579.1), Papio anubis (MG787545.1), Macaca mulatta (NC005943.1), Cercopithecus diana (NC023963.1), Leontopithecus chrysopygus (NC037878.1), Callimico goeldii (NC024628.1), Callithrix jacchus (CM021961.1), Trachypithecus poliocephalus (NC034795.1). Mus musculus (MT410886.1) was set as outgroup taxon.

Figure 2. Phylogenetic relationship of 17 species of Cercopithecus based on Maximum likelihood model. Genbank accession number: C. erythrotis (JQ256936.1), C. cephus (JQ256948.1), C. petaurista (JQ256983.1), C. ascanius (JQ256938.1), C. lhoesti (KJ434957.1), C. nictitans (JQ256951.1), C. albogularis (NC021944.1), C. mitis (KJ434956.1), C. doggetti (JQ256953.1), C. hamlyni (JQ256924.1), C. diana (KJ434958.1), C.roloway (JQ256923.1), C. neglectus (MW160353), C. neglectus (JQ256930.1), C. mona (JQ256979.1), C. pogonias (JQ256989.1), C. wolfi (JQ256984) and C. denti (JQ256988.1). Macaca mulatta (NC005943.1) was set as outgroup taxon.

Data availability statement

The genome sequence data that support the findings of this study are openly available in GenBank of NCBI at (https://www.ncbi.nlm.nih.gov/) under the accession no. MW160353 and available at: https://www.ncbi.nlm.nih.gov/nuccore/MW160353.1/.