Genetic structure of Culex tritaeniorhynchus (Diptera: Culicidae) based on COI DNA barcodes

Figures & data

Figure 1. Sampling sites of 21 Culex tritaeniorhynchus geographic populations in the mainland of China.

Table 1. Genetic diversity of 21 geographic populations based on COI barcode.

Samples sites	n	H	S	Hd	p	K
BJ	15	13	40	0.971	0.00974	6.40952
CQ	6	6	40	1.000	0.3040	20.00000
FJ	13	13	23	1.000	0.00758	4.98718
GD	5	4	9	0.900	0.00547	3.60000
GS	12	11	46	0.985	0.02888	19.00000
GX	24	19	52	0.960	0.01228	8.07971
GZ	29	26	57	0.990	0.02307	15.18227
HEB	15	13	46	0.981	0.02841	18.6954
HUB	7	5	8	0.905	0.00463	3.04762
HAN	24	20	33	0.964	0.00651	4.28261
HUN	38	29	63	0.979	0.02603	17.13087
JS	27	22	52	0.983	0.02736	18.00285
JX	15	11	51	0.905	0.02908	19.13333
HEN	48	36	59	0.973	0.01973	12.98138
NX	13	11	40	0.962	0.02326	15.30769
SC	39	32	53	0.970	0.02358	15.51552
SH	29	19	64	0.904	0.03007	19.78818
SX	20	18	50	0.989	0.02442	16.06842
LN	57	46	67	0.984	0.02016	13.26504
YN	23	23	53	1.000	0.01379	9.07115
ZJ	26	23	58	0.982	0.02253	14.82462

n: Number of samples; H: Number of haplotypes; S: Number of variable sites; Hd: Haplotypes diversity; p: Nucleotide diversity; K: Average number of nucleotide differences.

Figure 2. Neighbor-joining tree based on COI barcodes region of C. tritaeniorhynchus. Bootstrap support (1,000 replicates) of nodes from NJ tree and ML tree are indicated above and below the branches, respectively. Only nodes with BS > 70% are labeled.

Figure 3. Haplotype network based on COI barcodes of Culex tritaeniorhynchus. Node sizes are proportional to haplotype frequencies. Lines linking the nodes are proportional to the mutation steps.

Table 2. AMOVA analysis of C. tritaeniorhynchus populations based on COI gene fragments.

Source of variation	d.f.	Sum of squares	Variance components	Percentage of variation
Among groups	3	2.461	0.00276Va	0.56
Among populations within groups	17	9.455	0.00315Vb	0.64
Within populations	464	225.229	0.48541Vc	98.80
Total	484	237.144	0.49132
Fixation Indices	Fst = 0.01203, Nm = 47.11

Data availability statement

The data that support the findings of this study are available in the National Center for Biotechnology Information (NCBI) at [https://www.ncbi.nlm.nih.gov/], reference number [MW488441-MW488925]