Characterization of the complete mitochondrial genome of Omosita colon (Coleoptera: Nitidulidae)

Figures & data

Figure 1. Omosita colon on a highly decomposed pig carcass in the field (Suzhou, China).

Table 1. GenBank accession numbers of published infraorder Cucujiformia members and outgroup.

Infraorder	Family	Species	Accession number
Cucujiformia	Nitidulidae	Carpophilus dimidiatus	MN604384
		Carpophilus pilosellus	MN604383
		Aethina tumida	MF943248
		Epuraea guttata	KX087289
	Cerambycidae	Anastrangalia sequensi	KY773687
		Cortodera humeralis	KX087264
		Monochamus alternatus	KJ809086
	Meloidae	Hycleus phaleratus	MF491389
		Hycleus marcipoli	KX161857
		Hycleus cichorii	MF491388
		Epicauta ruficeps	MN913338
	Tenebrionidae	Blaps rhynchoptera	MN267802
		Tribolium audax	KJ752724
		Tribolium castaneum	KM009121
Scirtiformia	Eucinetidae	Eucinetus haemorrhoidalis	KX035155

Figure 2. The mitogenome arrangement of O. colon. The red color is transfer RNA (tRNA) genes, the green is protein-coding genes (PCGs), and the orange is ribosomal RNA (rRNA) genes.

Table 2. Organization of the O. colon mitogenome.

Gene	Position	Length (bp)	Intergenic sequence (bp)	Start codon	Stop codon	Strand
trnI(gau)	1233–1296	64	−3	–	–	+
trnQ(uug)	1294–1362	69	−1	–	–	−
trnM(cau)	1362–1430	69	−15	–	–	+
ND2	1416–2435	1020	−2	ATC	TAA	+
trnW(uca)	2434–2498	65	5	–	–	+
trnC(gca)	2504–2565	62	4	–	–	−
trnY(gua)	2570–2636	67	−8	–	–	−
CO I	2629–4173	1545	−5	ATT	TAA	+
trnL1(uaa)	4169–4233	65	0	–	–	+
CO II	4234–4921	688	0	ATT	T	+
trnK(cuu)	4922–4991	70	0	–	–	+
trnD(guc)	4992−5055	64	0	–	–	+
ATP8	5056−5211	156	−7	ATT	TAG	+
ATP6	5205−5879	675	−1	ATG	TAA	+
CO III	5879−6666	788	−1	ATG	TA	+
trnG(ucc)	6666−6730	65	0	–	–	+
ND3	6731−7084	354	6	ATT	TAA	+
trnA(ugc)	7091−7156	66	0	–	–	+
trnR(ucg)	7157−7221	65	−1	–	–	+
trnN(guu)	7221−7284	64	0	–	–	+
trnS1(ucu)	7285−7351	67	0	–	–	+
trnE(uuc)	7352−7416	65	0	–	–	+
trnF(gaa)	7417−7483	67	9	–	–	−
ND5	7493−9197	1705	0	ATT	T	−
trnH(gug)	9198−9261	64	−1	–	–	−
ND4	9261−10,588	1328	−7	ATG	TA	−
ND4L	10582−10,869	288	2	ATG	TAA	−
trnT(ugu)	10,872−10,936	65	0	–	–	+
trnP(ugg)	10,937−11,002	66	1	–	–	−
ND6	11,004−11,510	507	−1	ATT	TAA	+
CYTB	11,510−12,649	1140	–2	ATG	TAG	+
trnS2(uga)	12,648−12,715	68	17	–	–	+
ND1	12,733−13,683	951	1	ATT	TAG	−
trnL2(uag)	13,685−13,751	67	−38	–	–	−
l-rRNA	13,714−15,047	1334	–2	–	–	−
trnV(uac)	15,046−15,114	69	−2	–	–	−
s-rRNA	15,113−15,899	787	/	–	–	−

Intergenic sequence represents (+) values as intergenic nucleotides and (–) values as overlapping regions.

Table 3. Nucleotide composition of O. colon mitogenome.

Feature	A + T (%)	AT-skew	G + C (%)	GC-skew
Mitogenome	79.8	0.029	20.2	–0.178
PCGs	78.0	−0.140	22.0	–0.010
tRNA genes	79.5	0.030	20.5	0.154
rRNA genes	82.0	−0.059	18.0	0.333

Figure 3. Relative synonymous codon usage (RSCU) for the O. colon mitogenome.

Figure 4. Putative secondary structures of tRNAs found in the mitogenome of O. colon.

Figure 5. Molecular phylogenetic analysis of infraorder Cucujiformia by ML method based on complete mitogenomes.

Data availability statement

Mitogenome data supporting this study are openly available in GenBank at: https://www.ncbi.nlm.nih.gov/nuccore/MT749275. Associated BioProject, SRA, and BioSample accession numbers are https://www.ncbi.nlm.nih.gov/bioproject/PRJNA670574 https://www.ncbi.nlm.nih.gov/sra/SRR12879478 and SAMN16513378, respectively.