1,044
Views
0
CrossRef citations to date
0
Altmetric
Innovation in Biomedical Science and Engineering

In vivo MR imaging of folate-receptor expression with the folate-specific nanospheres in a C6 glioblastoma model

, , , &

Figures & data

Figure 1. In vivo T2-MR images C6 tumor-bearing mice at designated time points after administration with (a) FA-targeted nanospheres, (b) non-FA-targeted nanospheres, and (c) FA-inhibited nanospheres. The tumor sites were highlighted with red circles.

Figure 1. In vivo T2-MR images C6 tumor-bearing mice at designated time points after administration with (a) FA-targeted nanospheres, (b) non-FA-targeted nanospheres, and (c) FA-inhibited nanospheres. The tumor sites were highlighted with red circles.

Figure 2. T2 relaxation time of the tumor regions in the groups treated with FA-targeted, non-FA-targeted, and FA-inhibited nanospheres at the specified time points. All values are presented as a mean ± SD (n = 3), *: P < 0.05.

Figure 2. T2 relaxation time of the tumor regions in the groups treated with FA-targeted, non-FA-targeted, and FA-inhibited nanospheres at the specified time points. All values are presented as a mean ± SD (n = 3), *: P < 0.05.

Figure 3. Prussian blue staining results of the tumor tissues in the groups intervened with FA-MAN (a), MAN (b) and FA-MAN + FA (c) at 72 h post-injection (×400 magnification) (the arrows show the Prussian blue-stained iron nanoparticles).

Figure 3. Prussian blue staining results of the tumor tissues in the groups intervened with FA-MAN (a), MAN (b) and FA-MAN + FA (c) at 72 h post-injection (×400 magnification) (the arrows show the Prussian blue-stained iron nanoparticles).

Figure 4. Changes in body weight of mice as a function of one month after intravenous injection of FA-MAN/MAN.

Figure 4. Changes in body weight of mice as a function of one month after intravenous injection of FA-MAN/MAN.

Figure 5. In vivo toxicology and serum sample biochemcal examination data acquired from BALB/c mice after 30 days post-injection intravenously with 150 µL physiological saline of FA-MAN/MAN nanospheres (10 mg Fe/ml): Blood routine test contained white blood cells (WBC), red blood cells (RBC) and hemoglobin (HGB); hepatic function biomarkers included alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP); renal function parameters included blood urea nitrogen (BUN) and creatinine (CREA). Untreated healthy mice were used as the negative control. Five mice were included in each group.

Figure 5. In vivo toxicology and serum sample biochemcal examination data acquired from BALB/c mice after 30 days post-injection intravenously with 150 µL physiological saline of FA-MAN/MAN nanospheres (10 mg Fe/ml): Blood routine test contained white blood cells (WBC), red blood cells (RBC) and hemoglobin (HGB); hepatic function biomarkers included alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP); renal function parameters included blood urea nitrogen (BUN) and creatinine (CREA). Untreated healthy mice were used as the negative control. Five mice were included in each group.

Figure 6. H&E staining iamges of the major organs (heart, liver, spleen, lung, kidney) from different groups. Mice were intravenously injected with 150 µL physiological saline of FA-MAN/MAN nanospheres (10 mg Fe/ml) and dissected after 30 days of post-injection (Scale bar: 50 µm).

Figure 6. H&E staining iamges of the major organs (heart, liver, spleen, lung, kidney) from different groups. Mice were intravenously injected with 150 µL physiological saline of FA-MAN/MAN nanospheres (10 mg Fe/ml) and dissected after 30 days of post-injection (Scale bar: 50 µm).