Limited diversity of epibiont bdelloid rotifers and no pattern of codiversification with the highly diverse endemic amphipods of a coastal zone of Lake Baikal

Figures & data

Table I. Information on samples depending on substrate (epibiont on amphipod, with number of analysed individuals in parentheses; or on other invertebrates; or from the environment), host species in alphabetical order for epibiont samples, sample ID with sampling date, depth, and coordinates (WGS84 reference system). For each sample, occurrence of bdelloid species is reported, with the number of animals for the focal species Embata parasitica, and the number of sequenced individuals in parentheses.

Substrate	Species/habitat	Sample ID	Date	Depth (m)	Latitude	Longitude	Dissotrocha aculeata (Ehrenberg, 1832)	Embata parasitica (Giglioli, 1863)	Habrotrocha collaris (Ehrenberg, 1832)	Philodina citrina Ehrenberg, 1830	Philodina flaviceps Bryce, 1906	Philodina roseola Ehrenberg, 1832	Philodina sp. 1	Philodina sp. 2	Pleuretra sulcata Bartoš, 1950	Rotaria rotatoria (Pallas, 1766)
Amphipods	Brandtia latissima lata (Dybowsky, 1874) (7)	BA009	9 July 2016	0.5	51.903137	105.068853
Amphipods	Brandtia sp. (5)	BA001b	9 July 2016	5	51.902904	105.069187
Amphipods	Brandtia sp. (8)	BA002b	10 July 2016	0.5	51.902692	105.067431
Amphipods	Eulimnogammarus cyaneus (Dybowsky, 1874) (6)	BA012	9 July 2016	0.5	51.903137	105.068853		13 (5)
Amphipods	Eulimnogammarus maackii (Gerstfeldt, 1858) (2)	BA006	9 July 2016	0.5	51.903137	105.068853
Amphipods	Eulimnogammarus marituji Bazikalova, 1945 (12)	BA011	9 July 2016	0.5	51.903137	105.068853		15 (6)
Amphipods	Eulimnogammarus verrucosus (Gerstfeldt, 1858) (10)	BA008	9 July 2016	0.5	51.903137	105.068853		3 (2)
Amphipods	Eulimnogammarus viridis (Dybowsky, 1874) (8)	BA007	9 July 2016	0.5	51.903137	105.068853
Amphipods	Eulimnogammarus vittatus (Dybowsky, 1874) (1)	BA010	9 July 2016	0.5	51.903137	105.068853		1 (1)
Amphipods	Gmelinoides fasciatus (Stebbing, 1899) (7)	BA013	9 July2016	0.5	51.903137	105.068853		63 (41)
Amphipods	Hyalellopsis sp. (8)	BA002c	10 July 2016	0.5	51.902692	105.067431
Amphipods	Micruropus wohlii (Dybowsky, 1874) (13)	BA004	9 July 2016	0.5	51.903137	105.068853
Amphipods	Ommatogammarus sp. (3)	BA018	10 July 2016	20	51.901742	105.069092
Amphipods	Pallasea cancelloides (Gerstfeldt, 1858) (14)	BA005	9 July 2016	0.5	51.903137	105.068853
Amphipods	Pallasea sp. (7)	BA014	10 July 2016	20	51.901742	105.069092		3 (2)
Amphipods	Poekilogammarus sp. (6)	BA015	10 July 2016	20	51.901742	105.069092
Amphipods	Undetermined species (5)	BA016	10 July 2016	20	51.901742	105.069092
Amphipods	Undetermined species (6)	BA017	10 July 2016	20	51.901742	105.069092
Amphipods	Undetermined species (5)	BA019	10 July 2016	20	51.901742	105.069092
Amphipods	Undetermined species (5)	BA020	10 July 2016	20	51.901742	105.069092
Amphipods	Undetermined species (3)	BA022	10 July 2016	20	51.901742	105.069092
Amphipods	Undetermined species (3)	BA025	10 July 2016	30	51.90005	105.065992
Amphipods	Undetermined species (4)	BA026	10 July 2016	30	51.90005	105.065992
Other hosts	Trichoptera larvae	BA003	10 July 2016	0.5	51.902692	105.067431				x			x			x
Other hosts	Lubomirskia baikalensis (Pallas, 1776)	BA029	14 July 2016	7	51.860053	104.852487								x
Environment	Sponges and rocks from the sediment	BA001	9 July 2016	5	51.902904	105.069187			x						x
Environment	Macrophytes and sediments	BA002	10 July 2016	0.5	51.902692	105.067431				x		x				x
Environment	Algae, detritus, sediments	BA021	10 July 2016	20	51.901742	105.069092				x
Environment	Algae, detritus, sediments	BA023	9 July 2016	0.5	51.903137	105.068853				x	x
Environment	Algae, sediment, sponges	BA024	10 July 2016	30	51.90005	105.065992				x
Environment	Pond near the lake	BA027	12 July 2016	0.2	51.912482	105.074223	x
Environment	Pools at the shore	BA028	13 July 2016	0.2	51.903168	105.074099	x
Environment	Pools at the shore	BA029	13 July 2016	0.2	51.903168	105.074099				x

Figure 1. Phylogenetic reconstruction of the evolutionary relationships between all known and available COI sequences for the genus Embata according to maximum likelihood, with branch length proportional to the number of substitution sites of the scale bar. Support values, expressed as aLRT > 0.80, are reported above branches but not for short terminal branches. Samples downloaded from GenBank are reported with their GenBank accession number; samples from New Caledonia are marked with the prefix NC; samples from Baikal are marked with the prefix H for haplotypes of Embata parasitica, with the host amphipod species indicated for each clade; samples from Philodina citrina from Baikal, used as outgroup, are marked with the prefix h for haplotype. The number of individual animals having the same haplotype is reported in parentheses.

Data availability statement

All new sequences were deposited in GenBank under accession numbers OP033973–OP034029 for Embata parasitica from Lake Baikal, OP034030–OP034043 for Embata sp. from New Caledonia, and OP033962–OP033972 for Philodina citrina from Lake Baikal.