Comparison of the ICP OES viewing modes efficiency in the estimation of cadmium (Cd) and lead (Pb) in whole blood samples

Figures & data

Table

Time	Temperature	Energy (w)
15 min.	150 °C	1500 (Maxi.)
25 min.	150 °C	1500 (Maxi.)

Figure 1. Linearity data was obtained from a calibration curve that contained a standard solution of Cd and Pb.

Table 1. Correlation coefficient and regression equations for Cd and Pb.

Mode	Cd		Pb
	Correlation coefficient (r^2)	Regression equations	Correlation coefficient (r^2)	Regression equations
Axial	0.999	y = 26.198x − 19.5	0.999	y = 2.2034x + 9.2
Radial	0.999	y = 7.1624x − 3.7	0.993	y = 0.5388x + 6.3
Dual	Axial = 0.999	y = 32.097x − 43.9	Axial = 0.998	y = 2.6526x + 6.7
	Radial = 0.999	y = 8.2746x + 4.9	Radial = 0.999	y = 0.5631x + 12.1

Table 2. Recovery for standard toxic trace metals understudy.

Metal	Recovery %
	Axial viewing mode	Radial viewing mode	Dual axial and radial viewing mode
			Axial	Radial
Cd	92	92	88	89
Pb	89	89	87	84

Table 3 (a). Validation parameters.

Metal	Axial mode	Radial mode	Axial mode	Radial mode	Dual axial and radial mode
					Average (µg/ml) n = 24		% RSD^b n = 24
	Average^a (µg/ml) n = 24*		% RSD^b n = 24		Axial mode	Radial mode	Axial mode	Radial mode
Cd	56.9 ± 1.9	56.8 ± 2.1	3.4	3.7	55.2 ± 1.7	55.4 ± 1.6	3.1	2.9
Pb	55.7 ± 1.3	55.7 ± 3.8	2.3	6.8	53.8 ± 2.4	52.6 ± 3.2	4.5	6.1

* n = 24 for each spike level was evaluated in ten replicates.

^aAverage of the spike level.

^bRSD = coefficient of variation.

Table 3 (b). Validation parameters.

Metal	Axial mode	Radial mode	Axial mode	Radial mode	Dual axial and radial mode
					LOD (µg/l) n = 24		LOQ (µg/l) n = 24
	LOD (µg/l) n = 24		LOQ (µg/l) n = 24		Axial mode	Radial mode	Axial mode	Radial mode
Cd	51.9	47.3	78.9	143.3	24.8	10.3	75.0	31.2
Pb	33.7	20.7	102.1	62.8	35.5	12.7	107.7	38.5