Figures & data
Figure 1. The time-dependent changes of the algal densities with or without HA as affected by 0.5 mg/L of Cu (a) and variations of final growth rates (b) and Chl-a (c) of C. vulgaris under various Cu and HA concentrations. * and ** mean significantly (p < 0.05) and extremely significantly (p < 0.01), respectively
![Figure 1. The time-dependent changes of the algal densities with or without HA as affected by 0.5 mg/L of Cu (a) and variations of final growth rates (b) and Chl-a (c) of C. vulgaris under various Cu and HA concentrations. * and ** mean significantly (p < 0.05) and extremely significantly (p < 0.01), respectively](/cms/asset/d854df37-30b4-4897-89f6-6a4dfe536ee3/tcsb_a_1995504_f0001_oc.jpg)
Figure 2. The concentrations of superoxide dismutase (a) and malondialdehyde (b) in C. vulgaris after 96 h of exposure as affected by different Cu and HA concentrations. * and ** mean significantly (p < 0.05) and extremely significantly (p < 0.01), respectively
![Figure 2. The concentrations of superoxide dismutase (a) and malondialdehyde (b) in C. vulgaris after 96 h of exposure as affected by different Cu and HA concentrations. * and ** mean significantly (p < 0.05) and extremely significantly (p < 0.01), respectively](/cms/asset/4942dea7-6060-4928-8705-87e4b05ad92c/tcsb_a_1995504_f0002_oc.jpg)
Figure 3. The Cu concentration-dependent changes of proteins and polysaccharides in the EPS matrix in the absence and presence of HA after 96 h of exposure. (a) proteins in the LB-EPS fraction; (b) proteins in the TB-EPS fraction; (c) polysaccharides in LB-EPS fraction; and (d) polysaccharides in TB-EPS fraction. * and ** mean significantly (p < 0.05) and extremely significantly (p < 0.01), respectively
![Figure 3. The Cu concentration-dependent changes of proteins and polysaccharides in the EPS matrix in the absence and presence of HA after 96 h of exposure. (a) proteins in the LB-EPS fraction; (b) proteins in the TB-EPS fraction; (c) polysaccharides in LB-EPS fraction; and (d) polysaccharides in TB-EPS fraction. * and ** mean significantly (p < 0.05) and extremely significantly (p < 0.01), respectively](/cms/asset/c3ea4a55-fd5a-47de-b93d-eca037e38520/tcsb_a_1995504_f0003_oc.jpg)
Figure 4. The EEM contours of tryptophan-like component (C1) (a) and tyrosine-like component (C2) (b) and their variations in fluorescent scores in response to different Cu and HA concentrations
![Figure 4. The EEM contours of tryptophan-like component (C1) (a) and tyrosine-like component (C2) (b) and their variations in fluorescent scores in response to different Cu and HA concentrations](/cms/asset/c5305c3f-0c4e-4719-8804-aec838f029ca/tcsb_a_1995504_f0004_oc.jpg)
Figure 5. Variations in protein-like FIFFF fractograms and molecular weight distribution in response to Cu and HA. It was noted that (a) and (b) were the FIFFF fractograms for LB-EPS and TB-EPS fractions and (c) and (d) were the molecular weight distribution of protein-like substances for LB-EPS and TB-EPS fractions, respectively
![Figure 5. Variations in protein-like FIFFF fractograms and molecular weight distribution in response to Cu and HA. It was noted that (a) and (b) were the FIFFF fractograms for LB-EPS and TB-EPS fractions and (c) and (d) were the molecular weight distribution of protein-like substances for LB-EPS and TB-EPS fractions, respectively](/cms/asset/0e6d5f26-2f5e-45a5-8d87-89de6c6c96d4/tcsb_a_1995504_f0005_oc.jpg)