Cyanide degradation and antagonistic potential of endophytic Bacillus subtilis strain BEB1 from Bougainvillea spectabilis Willd

Figures & data

Figure 1. In vitro antagonistic activity of Bacillus subtilis BEB1 against Neocosmospora keratoplastica, Rhizoctonia solani and Stemphylium vesicarium. A) N. keratoplastica alone, B) N. keratoplastica + B. subtilis BEB1, C) R. solani alone, D) R. solani + B. subtilis BEB1, E) S. vesicarium alone, F) S. vesicarium + B. subtilis BEB1

Table 1. In vitro antagonistic activity of endophytic bacteria isolated from Bougainvillea spectabilis.

	Inhibition %
Bacterial isolate	Neocosmospora keratoplastica	Rhizoctonia solani	Stemphylium vesicarium
BEB1	51.0 a	24.6 a	56.3 a
BEB2	17.8 b	8.7 a	18.2 b
BEB3	25.0 ab	12.7 a	9.1 b
BEB5	39.2 ab	12.7 a	13.6 b
BEB6	28.6 ab	16.6 a	13.6 b

Data are mean of 3 replications.

Means with different letters are significantly (P ≤ 0.05) different.

Figure 2. Scanning electron micrographs showing morphological changes in the hyphae of Neocosmospora keratoplastica, Rhizoctonia solani and Stemphylium vesicarium due to antagonistic effect of Bacillus subtilis BEB1. A) N. keratoplastica alone, B) N. keratoplastica + B. subtilis BEB1, C) R. solani alone, D) R. solani + B. subtilis BEB1, E) S. vesicarium alone, F) S. vesicarium + B. subtilis BEB1.

Figure 3. Growth of Bacillus subtilis BEB1 at different concentrations of NaCN. The absorbance of the bacterial cultures was determined 72 h after inoculation. Values followed by different letters are significantly different according to LSD test (P < 0.05).

Figure 4. Degradation of NaCN by Bacillus subtilis BEB1. The cyanide content in the growth medium was determined 72 h after inoculation. Values followed by different letters are significantly different according to LSD test (P < 0.05).