Antihyperlipidemic and hepatic antioxidant effects of Leek leaf methanol extract in high fat diet-fed rats

Figures & data

Table 1. The both standard and high-fat diet formulation of rats.

Ingredients	Standard diet (grams)	High-fat diet (grams)
Casein, Lactic, 30 Mesh	200.00	200.00
Cystine, L	3.00	3.00
Starch, Corn	550.00	72.80
Lodex 10	150.00	100.00
Sucrose	4.00	176.80
Solka Floc, FCC200	50.00	50.00
Soybean Oil, USP	25.00	25.00
Fat (Butter oil)	20.00	177.5
Minerals (S10026B)	50.00	50.00
Choline Bitartrate	2.00	2.00
Vitamins (V10001C)	1.00	1.00
Dye	0.05	0.05

Table 2. GC-MS profile of phytochemical compounds of leek leaf methanolic extract.

Compound name	Compound nature	MW (g/mol)	RT (min)	Peak area (%)	Biological activity
Butanoic acid, 2-[(phenylmethoxy)imino]-, trimethylsilyl ester	Carboxylic acid ester	279.41	3.942	0.46	na
Hexadecanoic acid, methyl ester	Fatty acid ester	270.50	22.894	6.91	Antioxidant activity, nematicide, hypocholesterolemic, antiandrogenic,hemolytic (Easwaran and Ramani 2014; Kumar et al. 2014)
11,14,17-Eicosatrienoic acid, methyl ester	Fatty acid ester	320.50	24.151	1.82	na
9,12,15-Octadecatrienoic acid, methyl ester, (Z,Z,Z)-					Hepatoprotective, antihistaminic, hypocholesterolemic, antieczemic, anti-inflammatory* (Ganesh and Mohankumar 2017)
9,12,15-Octadecatrienoic acid, methyl ester, (Z,Z,Z)-	Fatty acid ester	292.50	24.288	0.63	*
9,12,15-Octadecatrienoic acid, methyl ester, (Z,Z,Z)-		292.50	24.373	0.55	*
9,12-Octadecadienoic acid (Z,Z)-, methyl ester	Fatty acid ester	294.50	24.553	4.38	Anticancer (Yu et al. 2005)
10,13-Octadecadienoic acid, methyl ester	Fatty acid ester	294.50			na
9,12,15-Octadecatrienoic acid, methyl ester, (Z,Z,Z)	Fatty acid ester	292.50	24.655	71.42	Antiinflammatory, Hypocholesterolemic, Anticancer, Hepatoprotective, Nematicide, Insectifuge, Antihistaminic, Antieczemic, 5-Alpha Reductase inhibitor, Antiandrogenic, Anticoronary. (Rehana and Nagarajan 2013)
11,13-Dimethyl-12-tetradecen-1-ol acetate	Alcohol	282.50	24.724	1.90	na
13-Oxabicyclo[10.1.0]tridecane	Cyclic hydrocarbon	182.30			na
Cyclohexanol, 5-methyl-2-(1-methyl ethyl)-, (1.alpha.,2.alpha.,5.alpha.)-	Cyclic alcohol	156.27			na
Octadecanoic acid, methyl ester	Fatty acid ester	298.00	24.809	1.91	Antimicrobial (Belakhdar et al. 2015)
Cyclotrisiloxane, hexamethyl-	Organosilicon	222.46	30.921	1.54	na
Cyclotrisiloxane, hexamethyl-	Organosilicon	222.46	31.101	0.69	na
Silane, 1,4-phenylenebis[trimethyl]	Hydride derivative	222.47	31.263	0.61	na
2,4,6-Cycloheptatrien-1-one, 3,5-bis-trimethylsilyl-	Ketone	250.48	31.819	1.37	na

MW, Molecular weight; RT, Retention time; na, No activity reported.

Figure 1. The effect of methanolic leek extract on body weight gain of normal and hyperlipidemic rats. Data are expresses as (mean ± SD, n = 6).

Figure 2. The effect of methanolic leek extract on food intake of normal and hyperlipidemic rats. Data are expresses as (mean ± SD, n = 6).

Figure 3. The effect of methanolic leek extract on lipid profile in serum of normal and hyperlipidemic rats. Data are expresses as (mean ± SD, n = 6). ^a: Control vs. HFD, ^b: HFD vs. other treatments.

Figure 4. The effect of methanolic leek extract on lipid profile in liver of normal and hyperlipidemic rats. Data are expresses as (mean ± SD, n = 6). ^a: Control vs. HFD, ^b: HFD vs. other treatments.

Figure 5. The effect of methanolic leek extract on hepatic enzymes of normal and hyperlipidemic rats. Data are expresses as (mean ± SD, n = 6). ^a: Control vs. HFD, ^b: HFD vs. other treatments.

Figure 6. The effect of methanolic leek extract on markers of oxidative stress of normal and hyperlipidemic rats. Data are expresses as (mean ± SD, n = 6). ^a: Control vs. HFD, ^b: HFD vs. other treatments.

Easwaran L, Ramani A. 2014. . phytochemical examination and gc-ms studies of the medicinal plant - Naravelia zeylanica. IJRDPL. 3(5):1180–1188.

 Google Scholar

Kumar SS, Samydurai P, Ramakrishnan R, Nagarajan N. 2014. Gas chromatography and mass spectrometry analysis of bioactive constituents of Adiantum capillus-veneris. Int J Pharm. 6(4):60–63.

 Google Scholar

Ganesh M, Mohankumar M. 2017. Extraction and identification of bioactive components in Sida cordata (Burm.f.) using gas chromatography-mass spectrometry. J Food Sci Technol. 54(10):3082–3091.

 Web of Science ®Google Scholar

Yu FR, Lian XZ, Guo HY, McGuire PM, Li R, Wang R, Yu F. 2005. Isolation and characterization of methyl esters and derivatives from Euphorbia kansui (Euphorbiaceae) and their inhibitory effects on the human SGC-7901 cells. J Pharm Pharm Sci. 8(3):528–535.

 PubMed Web of Science ®Google Scholar

Rehana BH, Nagarajan N. 2013. GC–MS determination of bioactive components of Wedelia chinensis (Osbeck) Merrill. J Chem Pharm. 5(4):279–285.

 Google Scholar

Belakhdar G, Benjouad A, Abdennebi EH. 2015. Determination of some bioactive chemical constituents from Thesium humile Vahl. J Mater Environ Sci. 6(10):2778–2783.

 Google Scholar