Non-oxidative effects of the flavonoid-rich fraction of Lasianthera africana leaves on human haemoglobin

Figures & data

Figure 1. Fresh leaves of Lasianthera africana P. Beauv.

Figure 2. Absorption spectrum of 800 µl of OxyHb (16 mM) in 10 Mm phosphate buffer at pH 7.6 after 3 h incubation at 25°C.

Figure 3. Absorption spectrum of 800 µl of 16 mM OxyHb in 10 Mm phosphate buffer at pH 7.6 with 100 µl of 5 mg/ml LAFRF for 3 h at 25°C.

Figure 4. Absorption spectrum of 800 µl of 16 mM OxyHb in 10 Mm phosphate buffer at pH 7.6 with 100 µl of 10 mg/ml LAFRF for 3 h at 25°C.

Figure 5. Absorption spectrum of 800 µl of 16 mM OxyHb in 10 Mm phosphate buffer at pH 7.6 with 100 µl of 20 mg/ml LAFRF for 3 h at 25°C.

Figure 6. Absorption spectrum of 800 µl of 16 mM OxyHb in 10 Mm phosphate buffer at pH 7.6 with 100 µl of 40 mg/ml LAFRF for 3 h at 25°C.

Figure 7. Absorption spectrum of 800 µl of 16 mM OxyHb in 10 Mm phosphate buffer at pH 7.6 with 100 µl of 80 mg/ml LAFRF for 3 h at 25°C.

Table 1. Absorbance at 541 and 576 nm and OxyHb concentration.

		Absorbance		OxyHb concentration
Groups	Tests	at 541 nm	at 576 nm	(mmol/l)
1	Normal OxyHb	0.321	0.302	0.019
2	OxyHb + 5 mg/ml LAFRF	0.425	0.442	0.027
3	OxyHb + 10 mg/ml LAFRF	0.427	0.444	0.028
4	OxyHb + 20 mg/ml LAFRF	0.475	0.493	0.031
5	OxyHb + 40 mg/ml LAFRF	0.443	0.470	0.029
6	OxyHb + 80 mg/ml LAFRF	0.443	0.475	0.029

Data availability statement

Due to the nature of this research, participants of this study did not agree for their data to be shared publicly. So supporting data is not available due to ethical concerns.