CircRNA_100920 enhanced oncolytic adenovirus therapy in colon cancer by suppressing IFN-γ expression

Figures & data

Figure 1. CircRNA_100920 was increased in CRC by using the bioinformatics analysis. A. Microarray data indicated that circRNA_100920 was significantly increased in CRC tissues. B. Box plots revealed normalized intensities from the cancerous and adjacent normal tissues.

Figure 2. Clinical significance of circRNA_100920 in indicating CRC progression. A. CircRNA_100920 was overexpressed in 80 pairs of cancerous tissues compared with adjacent normal tissues. B. This panel showed the Kaplan–Meier survival curve relative to circRNA_100920 expression levels. C. The receiver operating characteristic (ROC) curve analysis revealed circRNA_100920 could become a potential biomarker. *p<0.05.

Table 1. Relationship between clinical features and circRNA_100920 expression in CRC patients.

No. of Variables	cases	Low	High	P-value
Age (years)				0.412
<55	37	20	17
55	43	20	23
Gender				0.327
Male	44	21	23
Female	36	19	17
TNM stage				<0.05
I-II	39	25	14
III-IV	41	15	26
Tumor size				<0.05
<5 cm	35	24	11
5 cm	45	16	29
metastasis				<0.05
No	39	27	12
Yes	41	13	28

Figure 3. Knockdown of circRNA_100920 inhibited the cell proliferation in CRC cells. A. Relative expression of circRNA_100920 in CRC cell lines. B. Relative expression of circRNA_100920 in HCT8 and HCT116 cells transfected with si-circRNA_100980 and si-NC. C. Cell proliferation in CRC cell lines. D. Cell proliferation in HCT8 and HCT116 cells transfected with si-circRNA_100980 and si-NC. *p<0.05.

Figure 4. CircRNA_100920 enhanced oncolytic adenovirus therapy in CRC cells. A. Cell viability of si-circRNA_100920 group after the treatment of cisplatin (3 µg/ml) was lower than that of the si-NC group. B. Cell viability of HCT116 after the treatment of oncolytic adenovirus H101 (5 MOI) was lower than that of other CRC cells. C. Cell viability of si-circRNA_100920 group after the treatment of H101 (5 MOI) was higher than that of the si-NC group. *p<0.05.

Figure 5. CircRNA_100920 enhanced oncolytic adenovirus therapy in CRC cells by suppressing IFN-γ expression. A. Relative expression of IFN in HCT8 and HCT116 cells transfected with si-circRNA_100980 and si-NC. B. Relative expression of IFN-γ expression in 80 pairs of cancerous tissues and adjacent normal tissues. C. Spearman’s correlation analysis verified the negative correlation between circRNA_100920 expression and IFN-γ expression. D. Relative expression of IFN-γ in HCT8 and HCT116 cells transfected with si-circRNA_100980 and si- IFN-γ was detected by RT-qPCR assay. E. Relative expression of IFN-γ in HCT8 and HCT116 cells transfected with si-circRNA_100980 and si- IFN-γ was detected by Elisa assay. F. Knockdown of IFN-γ restored therapeutic effect of H101(5 MOI). *p<0.05.