Figures & data
Figure 1. The miR-125-5p is downregulated in osteosarcoma to promote sustained cell growth. (A) Expression of miR-125-5p in osteosarcoma and normal adjacent bone tissues (B) miR-125-5p expression in osteosarcoma cell lines (MG-63, Saos2 and HOS) and normal osteoblast cells, hFOB1.19 (C) overexpression of miR-125-5p in Saos2 cells (D) MTT assay for proliferation analysis of transfected Saos2 cells and (E) relative colony formation of miR-NC or miR-125 mimics transfected Saos2 cells. The experiments were performed in triplicate and expressed as mean ± SD (*P < .05).
Figure 2. The miR-125-5p induced autophagy in osteosarcoma cells. (A) AO staining of miR-NC or miR-125 mimics transfected Saos2 cancer cells (D) TEM study of miR-NC or miR-125 mimics transfected Saos2 cells (C) western blotting of beclin 1, p62, LC3B I and LCEB II proteins from transfected Saos2 cancer cells. The experiments were performed in triplicate.
Figure 3. The miR-125-5p reduced migration and invasion of osteosarcoma cells. (A) Scratch-heal migration assay of miR-NC or miR-125 mimics transfected Saos2 cells (B) transwell assay for quantitative analysis of miR-NC or miR-125 mimics transfected Saos2 cell invasion. The experiments were performed in triplicate and expressed as mean ± SD (*P < .05).
Figure 4. The miR-125-5p targets KLF13 in osteosarcoma. (A) Bio-informatics-based prediction of miR-125-5p regulatory target (B) dual-luciferase reporter assay for the interaction analysis of miR-125-5p with KLF13 (C) transcript level of KLF13 in osteosarcoma and normal bone tissues (D) KLF13 transcript level in osteosarcoma cell lines (MG-63, Saos2 and HOS) and normal osteoblast cells, hFOB1.19 (E) KLF13 protein expression in miR-125-5p overexpressing Saos2 cancer cells and negative control cells (F) MTT assay for proliferation analysis of si-NC or si-KLF13 transfected Saos2 cells (G) analysis of proliferation of Saos2 cancer cells transfected with miR-125-5p mimics, miR-125-5p mimics plus pcDNA-KLF13 or miR-125-5p negative control (miR-NC). The experiments were performed in triplicate and expressed as mean ± SD (*P < .05).
