Analysis of gut-associated fungi from Chinese mitten crab Eriocheir sinensis

Figures & data

Figure 1. Krona chart indicating the taxonomic identification and relative abundance of fungi isolated from gut samples of Chinese mitten crab.

Figure 2. Fungal genera obtained from gut samples of Chinese mitten crab.

Table 1. Fungal diversity molecularly detected in the gut of Chinese mitten crab.

Strain no.	Host	GenBank code	Best BLAST matches	Accession code	Max score	Overlap length	Query cover	E value	% match
1	M 1	MW958029.1	Aspergillus niger	MT729934.1	1027	1027	97%	0	99.30
2	M 1	MW958030.1	Aspergillus awamori	HM037957.1	1014	1014	97%	0	99.46
3	M 1	MW958031.1	Aspergillus niger	MT218381.1	1009	1093	97%	0	99.82
4	M 1	MW958032.1	Aspergillus tubingensis	MG551279.1	1020	1348	98%	0	99.47
5	M 1	MW958033.1	Aspergillus niger	MG759551.1	1035	1035	97%	0	99.47
6	M 1	MW958034.1	Penicillium sp.	KX008632.1	981	981	98%	0	99.45
7	M 1	MW958035.1	Aspergillus tubingensis	MH237653.1	1013	1013	98%	0	99.82
8	M 1	MW958036.1	Pyrenochaetopsis indica	MH410656.1	867	867	93%	0	99.38
9	M 1	MW958037.1	Aspergillus niger	MT597435.1	1038	1038	99%	0	98.80
10	M 1	MW958038.1	Talaromyces verruculosus	MN429191.1	987	987	97%	0	99.09
11	M 1	MW958039.1	Cladosporium perangustum	KF706664.1	915	915	99%	0	100.00
12	M 1	MW958040.1	Talaromyces verruculosus	MN429191.1	994	994	96%	0	99.28
13	M 1	MW958041.1	Aspergillus niger	KX099623.1	1018	1018	99%	0	99.29
14	M 2	MW958042.1	Penicillium sp.	KX008632.1	965	965	98%	0	99.81
15	M 2	MW958043.1	Talaromyces verruculosus	MN429191.1	994	994	97%	0	99.28
16	M 2	MW958044.1	Candida intermedia	DQ665263.1	623	623	99%	2e–174	99.42
17	M 2	MW958045.1	Fusarium incarnatum	MN853391.1	894	894	99%	0	100.00
18	M 2	MW958046.1	Talaromyces verruculosus	MN429191.1	1003	1003	96%	0	99.82
19	M 2	MW958047.1	Talaromyces funiculosus	MN429201.1	987	987	94%	0	100.00
20	M 2	MW958048.1	Talaromyces funiculosus	MH590622.1	977	977	96%	0	99.08
21	M 2	MW958049.1	Penicillium sp.	MN093874.1	970	970	99%	0	99.26
22	M 3	MW958050.1	Phoma eupyrena	KJ686363.1	870	870	97%	0	98.97
23	F 1	MW958051.1	Paraconiothyrium sp.	MF185997.1	987	987	92%	0	99.45
24	F 1	MW958052.1	Trichosporon coremiiforme	KY105733.1	911	911	98%	0	99.02
25	F 1	MW958053.1	Clavispora lusitaniae	MT731390.1	645	645	98%	0	99.72
26	F 1	MW958054.1	Aspergillus niger	MT446087.1	1031	1031	99%	0	99.47
27	F 1	MW958055.1	Penicillium sp.	MN093874.1	974	974	97%	0	99.44
28	F 1	MW958056.1	Acremonium sp.	MK541538.1	869	869	98%	0	99.79
29	F 1	MW958057.1	Mucor circinelloides	KX349461.1	1081	1081	99%	0	99.01
30	F 2	MW958058.1	Acremonium furcatum	LR026793.1	872	872	92%	0	98.58
31	F 2	MW958059.1	Aspergillus flavus	MT584825.1	712	712	88%	0	92.59
32	F 2	MW958060.1	Meyerozyma caribbica	KU200440.1	1042	1042	99%	0	99.65
33	F 2	MW958061.1	Trichoderma harzianum	KU375456.1	1044	1044	96%	0	99.65
34	F 2	MW958062.1	Pyrenochaetopsis sp.	LT592946.1	876	876	97%	0	98.79
35	F 2	MW958063.1	Penicillium sp.	MN093874.1	981	981	97%	0	99.63
36	F 2	MW958064.1	Pyrenochaetopsis sp.	LT592946.1	872	872	97%	0	99.58
37	F 2	MW958065.1	Alternaria alternata	MT446076.1	893	893	96%	0	96.65
38	F 2	MW958066.1	Clavispora lusitaniae	MT731390.1	625	625	96%	6e–175	99.42
39	F 3	MW958067.1	Penicillium sp.	KM222497.1	974	1239	98%	0	99.08
40	F 3	MW958068.1	Penicillium terrigenum	MH864538.1	974	974	95%	0	98.73
41	F 3	MW958069.1	Fusarium verticillioides	MK392032.1	952	952	96%	0	99.62

Notes: BLAST search closest matches of fungal internal transcribed spacer DNA sequences amplified from E. sinensis. Sample GenBank accession codes, accession codes for the closest GenBank matches, sequence identity, and overlap of each match are reported.

Figure 3. Taxonomic profiles of gut fungal communities at different taxonomic levels. (a) phylum; (b) class; (c) family; and (d) genus.

Figure 4. Venn diagram of the culturable fungal genera from female and male crabs.

Table 2. Indexes of alpha diversity of fungal communities associated with the gut of Chinese mitten crab.

	Shannon (H′)	Pielou (E)	Simpson (D)	Margalef (DMG)
Female	2.871	0.9934	0.005847	5.774
Male	2.347	0.9150	0.07792	3.882

Data availability statement

The Fungal DNA sequences amplified during this study are available in GenBank (https://blast.ncbi.nlm.nih.gov/Blast.cgi) under accessions MW958029–MW958069.