Identification of potential genes correlated with breast cancer metastasis and prognosis

Figures & data

Figure 1. Volcano plot of differentially expressed genes (DEGs). The red dots represent the upregulated DEGs and the blue dots indicate the downregulated DEGs.

Figure 2. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses of survival-related genes. The black line denotes the p value and the length of the bars shows the number of genes.

Figure 3. The protein-protein interaction (PPI) network and sub-module network of survival-related genes. (A) PPI network; (B) significantly enriched sub-module network. The green circular nodes represent the downregulated genes.

Table 1. Top 15 hub genes in protein-protein interaction and sub-module network.

PPI network		Sub-module network
Gene symbol	Degree	Gene symbol	Degree
CD79B	22	CD27	21
CXCL9	22	TBX21	21
CCL5	22	CD2	21
CD27	21	CD3E	21
TBX21	21	SELL	20
CD1C	21	PRF1	20
CD2	21	CCR7	20
CD3E	21	ZAP70	20
KLRB1	20	CD247	20
SELL	20	IL2RB	20
PRF1	20	KLRK1	19
CCR7	20	CD5	19
ZAP70	20	LCK	19
CD247	20	CD8A	19
IL2RB	20	GZMA	18

Figure 4. Transcriptional regulatory network. The green circles represent the downregulated genes; the purple triangles represent the miRNAs; and the yellow hexagons represent the transcription factors.

Table 2. The interaction pairs among miRNAs, transcription factors and genes.

Regulators	Targets	Regulators	Targets
hsa-miR-1908	CD40LG	hsa-miR-1296	ITK
hsa-miR-1908	CD8A	hsa-miR-1296	TBX21
hsa-miR-1908	TBX21	hsa-miR-1296	CCR7
hsa-miR-1908	IL2RB	hsa-miR-1296	CD247
hsa-miR-1908	CD27	hsa-miR-1296	IL2RG
hsa-miR-1908	CCR7	hsa-miR-3193	CD247
hsa-miR-1908	PDCD1	hsa-miR-3193	PDCD1
hsa-miR-663	CD40LG	hsa-miR-3193	IL2RG
hsa-miR-663	CD8A	hsa-miR-492	ZAP70
hsa-miR-663	TBX21	hsa-miR-492	ITK
hsa-miR-663	IL2RB	hsa-miR-492	CD5
hsa-miR-663	CD27	hsa-miR-492	IL2RB
hsa-miR-663	CCR7	hsa-miR-492	PDCD1
hsa-miR-663	PDCD1	BCL11A_GM12878_hg19	LCK
hsa-miR-3677-3p	TBX21	BCL11A_GM12878_hg19	IL2RB
hsa-miR-3677-3p	IL2RB	BCL11A_GM12878_hg19	CCR7
hsa-miR-3677-3p	CCR7	BCL11A_GM12878_hg19	PDCD1
hsa-miR-3677-3p	CD3E	–	–

Data availability

The data that support the findings of this study are publicly available in the GEO database (https://www.ncbi.nlm.nih.gov/geo/) under the accession number GSE102818.