Biodiversity screening of gut microbiome during the allogeneic hematopoietic stem cell transplantation: data from the real-life clinical practice

Figures & data

Table 1. Basic characteristics of patients in the study and their transplantation period (MRD, minimal residual disease).

Characteristic	Abs. number (n = 12)
Age, Median (25–75%)	34 years
Female	6
Primary disease
Acute lymphoblastic leukemia	4
Acute myeloid leukemia	4
Biphenotypic leukemia	2
Aplastic anemia	1
Chronic myelogenous leukemia	1
Patient status at the time of transplantation (for acute leukemia, n = 10)
Remission 1	7
Remission ⩾ 2	1
Relapse of disease	2
MRD status at the time of transplantation (for patients with acute leukemia in remission, n = 8)
MRD+	2
MRD−	6
Conditioning regimen
Myeloablative	2
Reduced intensity	10
Donor type
Related donor	3
Unrelated donor	9
Source of transplant
Peripheral blood	11
Bone marrow	1
Antibiotics during hospitalization^a
Carbapenems	9
Fluoroquinolones	3
Colistin	2
Cephalosporins	1
Vancomycin i/v	2
Linezolid	9
GVHD prophylaxis regimen
Cyclosporin+MTX	2
Tacrolimus + MMF	9
Cyclosporin + steroids (aplastic anemia)	1
In vivo T-cell depletion
Antithymocyte globulin	5
Post-transplant cyclophosphan	4
GVHD type
Acute	7
Chronic	2
GVHD localization
Skin	7
Mucosal membranes	1
Intestines	3
Liver	3
GVHD grade (NCCN)
Gr I	1
Gr II	4
Gr III	2
Gr IV	2
Median of observation	6

^a Antibacterial drugs were prescribed in combination and are not cumulative.

Figure 1. Biodiversity of intestinal microbiome samples depending on the main hematological disease (Leukemia - biphenotypic leukemia).

Figure 2. Biodiversity of intestinal microbiome samples during HSCT, in dynamics, depending on the sample number (1,2,3).

Figure 3. Dynamics of the composition of the intestinal microbiome at the taxonomic level of the type before HSCT (samples 1) and after HSCT (samples 2, 3).

Figure 4. Dynamics of the relative abundance of the 3 most common taxonomic phyla (Bacteroidetes, Firmicutes, Proteobacteria) of the intestinal microbiome before HSCT (samples 1) and after HSCT (samples 2, 3).

Data availability statement

The data that support the findings of this study are available on request from the corresponding author (I.S.). The data are not publicly available due to containing information that could compromise the privacy of research participants.