Key genes and regulatory networks of hypoxic preconditioning on osteoblasts

Figures & data

Figure 1. The top 20 differently expressed miRNAs in each two group.

Table 1. The target genes of the hypoxia preconditioning group compared with the long-time hypoxia group.

Target gene	miRNAs number (n)
Csf1r	43
Rnmt	42
Stard6	41
St8sia5	40
RGD1565222	39
Prrg4	38
Slc27a6	37
Ndufa2	36
Fam13b	35
Mmaa	34

Table 2. The target genes of the hypoxia preconditioning group compared with the control group.

Target gene	miRNAs number (n)
Tnnt2	31
Hk2	30
Cd24	29
Ube4b	28
Emp1	28
Sik3	27
Cacna2d1	27
Unc79	26
Dpysl4	26
Pknox2	25

Table 3. The target genes of long-time hypoxia group compared with control group.

Target gene	miRNAs number (n)
St3gal2	48
Nol3	47
Abcg5	46
Canx	45
Lgsn	44
Pola2	43
Gfy	42
Raver1	41
Slc16a3	41
Lca5	40

Figure 2. The top 10 BP, CC and MF of GO terms in the hypoxia preconditioning group compared with the long-time hypoxia group.

Figure 3. The top 10 terms of KEGG enrichment in the hypoxia preconditioning group compared with the long-time hypoxia group.

Figure 4. The top 10 BP, CC and MF of GO terms in the hypoxia preconditioning group compared with the control group.

Figure 5. The top 10 terms of KEGG enrichment in the hypoxia preconditioning group compared with the control group.

Figure 6. The top 10 BP, CC and MF of GO terms in the long-time hypoxia group compared with the control group.

Figure 7. The top 10 terms of KEGG enrichment in the long-time hypoxia group compared with the control group.

Figure 8. PPI networks. The color of genes gradually changed from purple to yellow as high scores to low scores based on the degree algorithm. (a) hypoxia preconditioning group compared with the long-time hypoxia group; (b): the hypoxia preconditioning group compared with the control group and (c): the long-time hypoxia group compared with the control group.

Figure 9. CCK-8 and cytokines detection results. Hypoxia preconditioning improved cell viability by CCK-8 assay (p < 0.01), enhanced ALP, IL10, and VEGF expression, and inhibited IL6 expression (p < 0.05), *p < 0.05, **p < 0.01. n = 20.

Data availability statement

The supplemental data used to support the findings of this study have been stored in Mendeley data repository (https://data.mendeley.com/datasets/6bx2p5zz84/1).