Improving photosynthesis and drought tolerance in Nicotiana tabacum L. by foliar application of salicylic acid

Figures & data

Figure 1. (a) Pn. (b) Tr. (c) Gs. (d) Ci. Pn: net photosynthesis rate; Gs: stomatal conductance; Tr: transpiration rate; Ci: intercellular CO₂ concentration. CK: water + Hoagland nutrient solution; D: water + 15% PEG6000-treated nutrient solutions; D + SA: 0.3 mmol/L SA + 15% PEG6000. Symbols of lower case letters indicate that the significant differences between treatments are at 0.05.

Figure 2. (a) SOD. (b) POD. (c) CAT. (d) MDA. (e) soluble protein. (f) proline. Superoxide dismutase (SOD); Peroxidase (POD); Catalase (CAT). Malondialdehyde (MDA). Symbols of lower case letters indicate that the significant differences between treatments are at 0.05.

Table 1. Statistics of transcriptome sequencing data.

Sample	Raw reads	Clean reads	Total mapped	Uniquely mapped	Q30(%)	GC content(%)
CK_1	82.58M	80.33M	77436684(96.40%)	69524574(86.55%)	95.82%	44.13%
CK_2	85.00M	82.70M	81159252(98.14%)	72641490(87.84%)	95.83%	44.01%
CK_3	90.07M	87.69M	86015985(98.09%)	77229420(88.07%)	95.90%	44.06%
D_1	90.62M	88.00M	86474855(98.27%)	78005814(88.64%)	95.78%	43.35%
D_2	90.81M	88.38M	86833722(98.25%)	78540519(88.87%)	95.87%	43.46%
D_3	85.46M	82.92M	81407742(98.18%)	73611775(88.78%)	95.70%	43.28%
D + S_1	88.61M	86.17M	84647498(98.24%)	76454307(88.73%)	95.86%	43.59%
D + S_2	92.20M	89.85M	88071017(98.01%)	79263049(88.21%)	95.98%	43.67%
D + S_3	86.96M	84.70M	83238969(98.28%)	74704930(88.20%)	95.85%	43.92%

Samples for mRNA-Seq derived from the different treatments. Q30: DNA sequencing quality ≥ 30. 1–3 represents three biological replications.

Figure 3. (a, b) The top 10 GO terms in biological process (BP), cellular component (CC), and molecular function (MF) enriched by differentially expressed genes between different treatments. (c, d) Enrichment analysis of DEGs.

Figure 4. (a). Venn diagram of DEGs between D-vs-CK down and D + S-vs-D up. (b). GO enrichment in DEGs response to SA. (c). KEGG pathway in DEGs response to SA.

Figure 5. (a) Expression level of genes related to photosynthesis; (b) Expression of genes related to photosynthesis-antenna proteins; (c) Gene expression related to porphyrin and chlorophyll metabolism; (d) Expression levels of genes related to carbon metabolism; (e) Expression pattern of genes related to starch and sucrose metabolism.

Data availability statement

The sequencing data were deposited in the National Center of Biotechnology Information database (https://www.ncbi.nlm.nih.gov/bioproject/PRJNA747725). The rest of the raw data that support the findings of this study are openly available in Science Data Bank at https://doi.org/10.57760/sciencedb.08474.