Evaluation of cytotoxic and genotoxic potential of avobenzone and octocrylene on human skin fibroblast cells

Figures & data

Figure 1. Cytotoxicity of AVB on human skin fibroblast cell line CCD-1118Sk. GC; untreated control, SC; solvent control (DMSO: 1.24% v/v), PC; positive control (500 µM H₂O₂). *: 0,05, ***P < 0.001 compared with SC.

Figure 2. Cytotoxicity of OCT on human skin fibroblast cell line CCD-1118Sk. GC; untreated control, SC; solvent control (dH₂O:1.5% v/v), PC; positive control (500 µM H₂O₂). *P < 0.05, **P < 0.01, ***P < 0.001 compared with SC.

Figure 3. Undamaged and damaged DNA observed by Comet assay (x 200). a: growth control b: solvent control (DMSO) c: solvent control (dH₂O) d: positive control (H₂O₂) e: IC₂₅ (A) f: IC₂₅ (O) g: IC₂₅ (A)+IC₂₅ (O).

Table 1. Comet values in DNA damage assay.

	Tail Length (µm) ±SD	Tail DNA % ±SD	OTM ± SD
GC	6.616 ± 0.916	4.157 ± 0.987	1.027 ± 0.439
SC (DMSO)	7.233 ± 0.7	6.716 ± 0.581	1.27 ± 0.175
SC (dH2O)	5.048 ± 0.81	2.712 ± 0.442	0.578 ± 0.249
PC (H2O2)	20.321 ± 3.109	11.542 ± 1.956	5.405 ± 1.261
IC 12.5 (A)	9.706 ± 1.312	7.291 ± 1.41	1.913 ± 0.575
IC 25 (A)	10.076 ± 1.631	4.821 ± 0.753	1.448 ± 0.395
IC 50 (A)	16.464 ± 2.862**	11.602 ± 2.158**	4.712 ± 1.275**
IC 12.5 (O)	13.288 ± 1.904**	9.264 ± 1.367**	3.002 ± 0.601**
IC 25 (O)	18.962 ± 3.071**	10.337 ± 1.823**	4.946 ± 1.35**
IC 50 (O)	22.443 ± 3.22**	17.356 ± 2.691**	7.551 ± 1.573**
IC 12.5 (A) + IC 12.5 (O)	14.183 ± 2.186 ##	15.01 ± 2.789 ##	4.78 ± 1.179 ##
IC 25 (A) + IC 25 (O)	26.257 ± 1.996 ++	22.927 ± 2.106 ++	7.107 ± 0.821 ++

Note: OTM: Olive tail moment; GC: untreated control; SC: solvent control; PC: positive control (150 µM H₂O₂); A: avobenzone; O: octocrylene. **P < 0.01 compared with SC (DMSO for A, dH₂O for O) ##: P < 0.01 compared with IC_12.5 A for tail length, compare with IC_12.5 A and O for tail DNA % and OTM. ++P < 0.01 compared with IC₂₅ A and O.

Figure 4. Intracellular ROS values. GC; untreated control, SC; solvent control, PC; positive control (100 µM H₂O₂), A; avobenzone, O; octocrylene, RFU; relative fluorescence unit, **P < 0.01 compared with SC (DMSO for A, dH₂O for O) ##P < 0.01 compared with IC_12.5 A and O. ++P < 0.01 compared with IC₂₅ A and O.

Data availability statement

The data that support the findings of this study are available in Mendeley data, V4 at www.doi.org/10.17632/224cwbbkk8.4.